کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8267168 | 1534944 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of endoplasmic reticulum stress and oxidative stress by vitamin D in endothelial cells
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کلمات کلیدی
CSPDn.s.tunicamycinJnk1SEAPeIF2αHBSSc-Jun N-terminal kinase 1MCP-1HUVECEB1089GAPDHHDLDMSO - DMSOhigh-density lipoprotein - HDL یا لیپوپروتئین با دانسیته بالا یا چگالی بالاSecreted alkaline phosphatase - آلکالن فسفاتاز تثبیت شدهDimethylsulfoxide - دیمتیل سولفواکسیدHuman umbilical vein endothelial cell - سلول اندوتلیالی ورید ناقل انسانیSuperoxide - سوپر اکسیدendoplasmic reticulum - شبکه آندوپلاسمی eukaryotic initiation factor 2α - عامل آغاز کننده یوکاریوتی 2αLow-density lipoprotein - لیپوپروتئین کم چگالی یا الدیال LDL - لیپوپروتئین کم چگالی(کلسترول بد)Hank's balanced salt solution - محلول نمک متعادل هانکnot significant - مهم نیستIntercellular adhesion molecule 1 - مولکول چسبندگی بین سلولی 1relative light units - واحدهای نسبی نورpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازglucose-regulated protein 78 - پروتئین تنظیم شده با گلوکز 78monocyte chemo-attractant protein-1 - پروتئین شیمیایی جذب مونوسیت-1Propidium iodide - پروتئین یدیدGrp 78 - گروپ 78glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Endoplasmic reticulum (ER) stress and oxidative stress promote endothelial dysfunction and atherosclerosis. Since vitamin D has been shown in several studies to lower the risk of cardiovascular disease, we examined the effects of vitamin D on ER stress and oxidative stress in endothelial cells. ER stress was measured using the placental secreted alkaline phosphatase assay and oxidative stress was measured by hydroethidine fluorescence. Expression of ER stress markers, including glucose-regulated protein 78, c-jun N-terminal kinase 1 phosphorylation, and eukaryotic initiation factor 2α phosphorylation, as well as X-box binding protein-1 splicing were measured in tunicamycin (TM)-treated human umbilical endothelial cells (HUVEC) treated with 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) and other vitamin D analogs. When TM and 1,25-(OH)2D3 were added simultaneously, 1,25-(OH)2D3 prevented ER stress. However, the effect was much stronger when cells were pre-treated with 1,25-(OH)2D3 for 24-h. However, ER stress was not inhibited by 25-OH vitamin D3 (25-OHD3) or the vitamin D analog EB1089. Both ZK191784 and the vitamin D metabolite 24,25-dihydroxyvitamin D3 were as effective as 1,25-(OH)2D3 in preventing ER stress. Similar effects were observed dextrose-induced stress. All of the compounds tested, except for 25-OHD3, inhibited dextrose-induced (27.5 mM) oxidative stress and ER stress. Although TM with and without 1,25-(OH)2D3 had no effect on VDR expression, inhibition of VDR expression via siRNA prevented 1,25-(OH)2D3, ZK191784, EB1089, and 24,25-dihydroxyvitamin D3 from inhibiting dextrose-mediated SO generation. Furthermore, each vitamin D analog, with the exception of 25-OHD3, prevented dextrose-induced toxicity. These results suggest that vitamin D has a protective effect on vascular endothelial cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 99, October 2016, Pages 1-10
Journal: Free Radical Biology and Medicine - Volume 99, October 2016, Pages 1-10
نویسندگان
Michael J. Haas, Mohammad Jafri, Kent R. Wehmeier, Luisa M. Onstead-Haas, Arshag D. Mooradian,