Development of a new application of the comet assay to assess levels of O6-methylguanine in genomic DNA (CoMeth)

► CoMeth is a modification of the comet assay to assess O6meG levels and study MGMT activity. ► O6meG is converted to strand breaks by MMR-proficient cells when MGMT is inhibited. ► CoMeth allows the study of new MGMT inhibitors and inducers of O6meG. ► CoMeth allows the study of drugs that act by demethylation of MGMT or MMR genes.