کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8293779 | 1536748 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid
ترجمه فارسی عنوان
تولید آنتیبادی های ضد الیگوساکارید که به شناسایی گلیکوپروتئین های پستانداران توسط ایمن سازی با یک گلیسوفسنگولایپید مصنوعی جدید
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کلمات کلیدی
مهندسی آنتی بادی، گلیکوسفینگولایپید، گلیکوپروتئین، اولیگوساکارید، سرامید، اسید چرب زنجیره ای بسیار طولانی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Here we report a new method for the efficient generation of antibodies that recognize the fine structures of oligosaccharides on glycoproteins. We found a newly designed artificial glycosphingolipid carrying a very long chain fatty acid to be a strong immunogen in mice, with the serum of immunized mice containing antibodies recognizing the oligosaccharide structure of the immunogen. First, we found that conjugation of a simple ceramide analogue to target oligosaccharides could enhance the immunogenicity of these oligosaccharides in these immunized mice. This effect was confirmed in mice immunized with the artificial glycosphingolipids carrying 6â²-Sialyl-LacNAc, 3â²-Sialyl-LacNAc and LacNAc. Next, we tried to improve the immunogenic enhancing effect of the ceramide analogue. In a model experiment using 6â²-Sialyl-LacNAc oligosaccharide, we manipulated the alkyl chains to several lengths, and found that a longer alkyl chain length of the fatty acid correlated with high immunogenicity. Among these we examined, artificial glycosphingolipids conjugated with a ceramide analogue carrying a very long chain fatty acid (lignoceric acid) showed the strongest immunogenicity. By using the artificial glycosphingolipid containing 6â²-Sialy-LacNAc and lignoceric acid, we succeeded in the generation of a new anti-6â²-Sialyl-LacNAc antibody that recognizes 6â²-Sialyl-LacNAc carrying glycoproteins but does not bind to 6â²-Sialyllactose, asialo-glycoporoteins and glycoproteins carrying 3â²-Sialyl-LacNAc. These results indicate that the established technology is valuable for the targeted generation of monoclonal antibodies against glycoproteins containing specific oligosaccharide structures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 4, 18 March 2018, Pages 983-989
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 4, 18 March 2018, Pages 983-989
نویسندگان
Tetsuya Okuda, Asami Fukui,