کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8293779 1536748 2018 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid
ترجمه فارسی عنوان
تولید آنتیبادی های ضد الیگوساکارید که به شناسایی گلیکوپروتئین های پستانداران توسط ایمن سازی با یک گلیسوفسنگولایپید مصنوعی جدید
کلمات کلیدی
مهندسی آنتی بادی، گلیکوسفینگولایپید، گلیکوپروتئین، اولیگوساکارید، سرامید، اسید چرب زنجیره ای بسیار طولانی،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Here we report a new method for the efficient generation of antibodies that recognize the fine structures of oligosaccharides on glycoproteins. We found a newly designed artificial glycosphingolipid carrying a very long chain fatty acid to be a strong immunogen in mice, with the serum of immunized mice containing antibodies recognizing the oligosaccharide structure of the immunogen. First, we found that conjugation of a simple ceramide analogue to target oligosaccharides could enhance the immunogenicity of these oligosaccharides in these immunized mice. This effect was confirmed in mice immunized with the artificial glycosphingolipids carrying 6′-Sialyl-LacNAc, 3′-Sialyl-LacNAc and LacNAc. Next, we tried to improve the immunogenic enhancing effect of the ceramide analogue. In a model experiment using 6′-Sialyl-LacNAc oligosaccharide, we manipulated the alkyl chains to several lengths, and found that a longer alkyl chain length of the fatty acid correlated with high immunogenicity. Among these we examined, artificial glycosphingolipids conjugated with a ceramide analogue carrying a very long chain fatty acid (lignoceric acid) showed the strongest immunogenicity. By using the artificial glycosphingolipid containing 6′-Sialy-LacNAc and lignoceric acid, we succeeded in the generation of a new anti-6′-Sialyl-LacNAc antibody that recognizes 6′-Sialyl-LacNAc carrying glycoproteins but does not bind to 6′-Sialyllactose, asialo-glycoporoteins and glycoproteins carrying 3′-Sialyl-LacNAc. These results indicate that the established technology is valuable for the targeted generation of monoclonal antibodies against glycoproteins containing specific oligosaccharide structures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 4, 18 March 2018, Pages 983-989
نویسندگان
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