کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8294788 | 1536754 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and functional characterization of a p-coumaroyl quinate/shikimate 3â²-hydroxylase from potato (Solanum tuberosum)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CGAHQTAntisense - آنتیسنسChlorogenic acid - اسید کلروژنیکempty vector - بردار خالیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدreverse transcription - رونویسی معکوسPotato - سیب زمینیMass spectrometry - طیف سنجی جرمیMuscle - عضلهRace - مسابقهmultiple sequence comparison by log-expectation - مقایسه توالی چندگانه با انتظارات منطقیwild type - نوع وحشیhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chlorogenic acid (CGA) plays an important role in protecting plants against pathogens and promoting human health. Although CGA accumulates to high levels in potato tubers, the key enzyme p-coumaroyl quinate/shikimate 3â²-hydroxylase (C3â²H) for CGA biosynthesis has not been isolated and functionally characterized in potato. In this work, we cloned StC3â²H from potato and showed that it catalyzed the formation of caffeoylshikimate and CGA (caffeoylquinate) from p-coumaroyl shikimate and p-coumaroyl quinate, respectively, but was inactive towards p-coumaric acid in in vitro enzyme assays. When the expression of StC3â²H proteins was blocked through antisense (AS) inhibition under the control of a tuber-specific patatin promoter, moderate changes in tuber yield as well as phenolic metabolites in the core tuber tissue were observed for several AS lines. On the other hand, the AS and control potato lines exhibited similar responses to a bacterial pathogen Pectobacterium carotovorum. These results suggest that StC3â²H is implicated in phenolic metabolism in potato. They also suggest that CGA accumulation in the core tissue of potato tubers is an intricately controlled process and that additional C3â²H activity may also be involved in CGA biosynthesis in potato.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 2, 5 February 2018, Pages 462-467
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 2, 5 February 2018, Pages 462-467
نویسندگان
Benjamin J. Knollenberg, Jingjing Liu, Shu Yu, Hong Lin, Li Tian,