Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris

► We isolated and characterized lipid droplets from P. pastoris for the first time. ► Lipidome and proteome analysis of P. pastoris lipid droplets were performed. ► Lipid droplets from P. pastoris are different from S. cerevisiae lipid droplets. ► P. pastoris lipid droplets contain much triacylglycerols but little steryl esters. ► A large number of P. pastoris lipid droplet proteins are involved in lipid metabolism.