کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8303866 | 1537956 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A low temperature-inducible protein AtSRC2 enhances the ROS-producing activity of NADPH oxidase AtRbohF
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کلمات کلیدی
ABART-PCRNADPH oxidase (NOX)NOx - NOXROS - ROSArabidopsis thaliana - آرابیدوپسیس تالیاناCold stress - استرس سردabscisic acid - اسید آبسزیکNADPH oxidase - اکسیداز NADPH Co-IP - شرکت-IPtransmembrane - فرابنفشCo-Immunoprecipitation - هم ایمن زداییreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسReactive oxygen species (ROS) - گونه های اکسیژن واکنشی (ROS)Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Reactive oxygen species (ROS) produced by NADPH oxidases play critical roles in plant environmental responses. Arabidopsis thaliana NADPH oxidase AtRbohF-mediated ROS-production is involved in abiotic stress responses. Because overproduction of ROS is highly toxic to cells, the activity of AtRbohF needs to be tightly regulated in response to diverse stimuli. The ROS-producing activity of AtRbohF is activated by Ca2Â + and protein phosphorylation, but other regulatory factors for AtRbohF are mostly unknown. In this study, we screened for proteins that interact with the N-terminal cytosolic region of AtRbohF by a yeast two-hybrid screen, and isolated AtSRC2, an A. thaliana homolog of SRC2 (soybean gene regulated by cold-2). A co-immunoprecipitation assay revealed that AtSRC2 interacts with the N-terminal region of AtRbohF in plant cells. Intracellular localization of GFP-tagged AtSRC2 was partially overlapped with that of GFP-tagged AtRbohF at the cell periphery. Co-expression of AtSRC2 enhanced the Ca2Â +-dependent ROS-producing activity of AtRbohF in HEK293T cells, but did not affect its phosphorylation-dependent activation. Low-temperature treatment induced expression of the AtSRC2 gene in Arabidopsis roots in proportion to levels of ROS production that was partially dependent on AtRbohF. Our findings suggest that AtSRC2 is a novel activator of Ca2Â +-dependent AtRbohF-mediated ROS production and may play a role in cold responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1833, Issue 12, December 2013, Pages 2775-2780
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1833, Issue 12, December 2013, Pages 2775-2780
نویسندگان
Tomoko Kawarazaki, Sachie Kimura, Ayako Iizuka, Shigeru Hanamata, Hitomi Nibori, Masataka Michikawa, Aya Imai, Mitsutomo Abe, Hidetaka Kaya, Kazuyuki Kuchitsu,