کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8320678 | 1539404 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential binding kinetics of replication protein A during replication and the pre- and post-incision steps of nucleotide excision repair
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کلمات کلیدی
LUDDDRFRAP6-4PPsFLIPCPDsssDNARPA6-4 photoproducts - 6-4 عکاسی محصولاتsingle stranded DNA - DNA تک رشته ایNER - DOWNDNA damage - آسیبDNAFluorescence loss in photobleaching - از دست دادن فلورسنت در برداشتن عینکDNA repair - ترمیم DNAnucleotide excision repair - تعمیر مجدد نوکلئوتیدیReplication stress - تنش تکراریAraC - حق العملCyclobutane pyrimidine dimers - دیررس پیریمیدین سیکلووبتانfluorescence recovery after photobleaching - فلوئورسانس پس از فوتوبلاسیکultraviolet light - نور ماوراءبنفش یا نور فرابنفشDNA replication - همانندسازی DNA، تکثیر DNAHydroxyurea - هیدروکسی اورهDNA damage response - واکنش به آسیب DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The ability of replication protein A (RPA) to bind single-stranded DNA (ssDNA) underlines its crucial roles during DNA replication and repair. A combination of immunofluorescence and live cell imaging of GFP-tagged RPA70 revealed that RPA, in contrast to other replication factors, does not cluster into replication foci, which is explained by its short residence time at ssDNA. In addition to replication, RPA also plays a crucial role in both the pre- and post-incision steps of nucleotide excision repair (NER). Pre-incision factors like XPC and TFIIH accumulate rapidly at locally induced UV-damage and remain visible up to 4Â h. However, RPA did not reach its maximum accumulation level until 3Â h after DNA damage infliction and a chromatin-bound pool remained detectable up to 8Â h, probably reflecting its role during the post-incision step of NER. During the pre-incision steps of NER, RPA could only be visualized at DNA lesions in incision deficient XP-F cells, however without a substantial increase in residence time at DNA damage. Together our data show that RPA is an intrinsically highly dynamic ssDNA-binding complex during both replication and distinct steps of NER.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 24, December 2014, Pages 46-56
Journal: DNA Repair - Volume 24, December 2014, Pages 46-56
نویسندگان
Audrey M. Gourdin, Loes van Cuijk, Maria Tresini, Martijn S. Luijsterburg, Alex L. Nigg, Guiseppina Giglia-Mari, Adriaan B. Houtsmuller, Wim Vermeulen, Jurgen A. Marteijn,