کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323235 | 1539890 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Post-transcriptional regulation of autophagy in C2C12 myotubes following starvation and nutrient restoration
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کلمات کلیدی
ULK1ATGForkhead box protein OBNIP3mTORC1THRLC3BPPIARPS6AMPKMAFbxRPLP0FIP200Vps34Muscle atrophy F-boxMuscle RING finger-1E3-ubiquitin ligaseCyclophilin-Ap70S6KUbiquitin–proteasomeACCDMEMDPBSmTORAMP-activated protein kinase - AMP-پروتئین کیناز فعال شده استDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoP/S - P / Sunc-51 like autophagy activating kinase 1 - unc-51 مانند فعال کردن kinase autophagy 1Akt/PKB - آکت / PKBacetyl-CoA carboxylase - استیل کروکسی سیلازSER - برای بودنanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancestandard error of the mean - خطای استاندارد میانگینmicrotubule-associated protein light chain 3 - زنجیره سبک پروتئینی مرتبط با میکروتوبول 3Serine - سرینSkeletal muscle - عضله اسکلتیFoxO - فاکسوDulbecco's phosphate-buffered saline - فسفات باسیل نمک DulbeccoSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیmammalian target of rapamycin - هدف پستانداران رپامایسینMammalian target of rapamycin complex 1 - هدف پستانداران مجتمع رپامایسین 1Proteolysis - پروتئولیزRibosomal protein S6 - پروتئین Ribosomal S6ribosomal protein S6 kinase - پروتئین ریبوزومی S6 کینازprotein kinase B - پروتئین کیناز Bpenicillin–streptomycin - پنی سیلین-استرپتومایسینautophagy-related gene - ژن مربوط به autophagy
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In skeletal muscle, autophagy is activated in multiple physiological and pathological conditions, notably through the transcriptional regulation of autophagy-related genes by FoxO3. However, recent evidence suggests that autophagy could also be regulated by post-transcriptional mechanisms. The purpose of the study was therefore to determine the temporal regulation of transcriptional and post-transcriptional events involved in the control of autophagy during starvation (4Â h) and nutrient restoration (4Â h) in C2C12 myotubes. Starvation was associated with an activation of autophagy (decrease in mTOR activity, increase in AMPK activity and Ulk1 phosphorylation on Ser467), an increase in autophagy flux (increased LC3B-II/LC3B-I ratio, LC3B-II level and LC3B-positive punctate), and an increase in the content of autophagy-related proteins (Ulk1, Atg13, Vps34, and Atg5-Atg12 conjugate). Our data also indicated that the content of autophagy-related proteins was essentially maintained when nutrient sufficiency was restored. By contrast, mRNA level of Ulk1, Atg5, Bnip3, LC3B and Gabarapl1 did not increase in response to starvation. Accordingly, binding of FoxO3 transcription factor on LC3B promoter was only increased at the end of the starvation period, whereas mRNA levels of Atrogin1/MAFbx and MuRF1, two transcriptional targets of FoxO involved in ubiquitin-proteasome pathway, were markedly increased at this time. Together, these data provide evidence that target genes of FoxO3 are differentially regulated during starvation and that starvation of C2C12 myotubes is associated with a post-transcriptional regulation of autophagy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 54, September 2014, Pages 208-216
Journal: The International Journal of Biochemistry & Cell Biology - Volume 54, September 2014, Pages 208-216
نویسندگان
Marine Maud Desgeorges, Damien Freyssenet, Stéphanie Chanon, Josiane Castells, Pascal Pugnière, Daniel Béchet, André Peinnequin, Xavier Devillard, Aurélia Defour,