کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323389 | 1539891 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased expression of prostaglandin reductase 1 in hepatocellular carcinomas from clinical cases and experimental tumors in rats
ترجمه فارسی عنوان
افزایش بیان پروستاگلاندین ردوکتاز 1 در کارسینوم های حاد سلولی از موارد بالینی و تومورهای تجربی در موش صحرایی
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کلمات کلیدی
NQO1NAD(P)H dehydrogenase, quinone 1GCLglutathione S-transferase Pi 1GSTs2AAFrRNALTB415d-PGJ2DenqRT–PCREPHXGSTP14HNEUGTsUDP glucuronosyltransferasesLXA4PGE2ENTTFAGGTmRNA15-deoxy-delta-12,14-prostaglandin J22-acetylaminofluorene - 2-استامینامین فلوئورن4-hydroxy-2-nonenal - 4-هیدروکسی-2 غیرنالcDNA - cDNAComplementary DNA - DNA تکمیلیH&E - H & EHCC - HCCMS/MS - MS / MSmessenger RNA - RNA messengerRibosomal RNA - RNA Ribosomalγ-Glutamyl transferase - γ-گلوتامیل ترانسفرازTrifluoroacetic acid - اسید Trifluoroaceticanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancediethylnitrosamine - دیاتیلنیتروستامینTandem mass spectra - طیف جرم دو جانبهLeukotriene B4 - لوکوترین B4lipoxin A4 - لیپوکین A4glutamate-cysteine ligase - لیگاز گلوتامات سیتئینTumor marker - نشانگر تومورhematoxylin-eosin - هماتوکسیلین ائوزینEpoxide hydrolase - هیدرولاز اپوکسیQuantitative reverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوس کمیProstaglandin E2 - پروستاگلاندین E2Hepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)glutathione S-transferases - گلوتاتیون S-transferases
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
To identify novel tumor-associated proteins, we analyzed the protein expression patterns from experimental hepatocellular carcinoma (HCC) that were induced using hepatocarcinogenesis models in rats. Rats were subjected to two previously described protocols of hepatocarcinogenesis using diethylnitrosamine as a carcinogen: the alternative Solt-Farber (aS&F) protocol, which induces HCC within 9 months, and Schiffer's model, which induces cirrhosis and multifocal HCC within 18 weeks. The patterns of protein expression from tumors and normal liver tissue were examined by SDS-PAGE and the bands identified at 33-34Â kDa were analyzed by mass spectrometry. The prostaglandin reductase 1 (PTGR1) showed the highest number of peptides, with a confidence of level >99%. The increased expression of PTGR1 in tumors was confirmed in these two models by Western blotting and by increase in alkenal/one oxidoreductase activity (25-fold higher than normal liver). In addition, the gene expression level of Ptgr1, as measured by qRT-PCR, was increased during cancer development in a time-dependent manner (200-fold higher than normal liver). Furthermore, PTGR1 was detected in the cytoplasm of neoplastic cells in rat tumors and in 12 human HCC cases by immunohistochemistry. These analyses were performed by comparing the expression of PTGR1 to that of two well-known markers of hepatocarcinoma, Glutathione S-transferase pi 1 (GSTP1) in rats and glypican-3 in humans. The increased expression and activity of PTGR1 in liver carcinogenesis encourage further research aimed at understanding the metabolic role of PTGR1 in HCC and its potential application for human cancer diagnosis and treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 53, August 2014, Pages 186-194
Journal: The International Journal of Biochemistry & Cell Biology - Volume 53, August 2014, Pages 186-194
نویسندگان
Ricardo Sánchez-RodrÃguez, Julia Esperanza Torres-Mena, Monica De-la-Luz-Cruz, Gloria Alejandra Bernal-Ramos, Saúl Villa-Treviño, Victoria Chagoya-Hazas, Luis Landero-López, Rebeca GarcÃa-Román, Patrick Rouimi, Luis Del-Pozo-Yauner,