کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323566 | 1539893 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
2-Arachidonoylglycerol modulates human endothelial cell/leukocyte interactions by controlling selectin expression through CB1 and CB2 receptors
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کلمات کلیدی
CB1sl-selectinSoluble L-selectinPSGL-1TNFReCBsCB2FAAHABHD62-arachidonoylglycerolECLmaglAPCDAPILPS2-AGPBSERKRT-PCR4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولMAPK - MAPKallophycocyanin - آلوفوکسیانینFatty acid amide hydrolase - اسید آمینه هیدرولاز اسید چربPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعSelectins - انتخاب هاendocannabinoids - اندوکانبایوئید هاenhanced chemiluminescence - بهبود شیمیایی لومنELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاtumour necrosis factor-α - تومور نکروز عامل αTNF-α - فاکتور نکروز توموری آلفاphycoerythrin - فایکوئیریدینPhosphate buffered saline - فسفات بافر شورmonoacylglycerol lipase - لیپاز monoacylglycerol لیپازlipopolysaccharide - لیپوپلی ساکاریدP-selectin glycoprotein ligand-1 - لیگاند گلیکوپروتئین P-selectin-1type-1 cannabinoid receptor - نوع 1 گیرنده کانابینوئیدReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیKinases - کینازها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Accumulated evidence points to a key role for endocannabinoids in cell migration, and here we sought to characterize the role of these substances in early events that modulate communication between endothelial cells and leukocytes. We found that 2-arachidonoylglycerol (2-AG) was able to initiate and complete the leukocyte adhesion cascade, by modulating the expression of selectins. A short exposure of primary human umbilical vein endothelial cells (HUVECs) to 2-AG was sufficient to prime them towards an activated state: within 1 h of treatment, endothelial cells showed time-dependent plasma membrane expression of P- and E-selectins, which both trigger the initial steps (i.e., capture and rolling) of leukocyte adhesion. The effect of 2-AG was mediated by CB1 and CB2 receptors and was long lasting, because endothelial cells incubated with 2-AG for 1 h released the pro-inflammatory cytokine tumour necrosis factor-α (TNF-α) for up to 24 h. Consistently, TNF-α-containing medium was able to promote leukocyte recruitment: human Jurkat T cells grown in conditioned medium derived from 2-AG-treated HUVECs showed enhanced L-selectin and P-selectin glycoprotein ligand-1 (PSGL1) expression, as well as increased efficiency of adhesion and trans-migration. In conclusion, our in vitro data indicate that 2-AG, by acting on endothelial cells, might indirectly promote leukocyte recruitment, thus representing a potential therapeutic target for treatment of diseases where impaired endothelium/leukocyte interactions take place.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 51, June 2014, Pages 79-88
Journal: The International Journal of Biochemistry & Cell Biology - Volume 51, June 2014, Pages 79-88
نویسندگان
Valeria Gasperi, Daniela Evangelista, Valerio Chiurchiù, Fulvio Florenzano, Isabella Savini, Sergio Oddi, Luciana Avigliano, Maria Valeria Catani, Mauro Maccarrone,