کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8324013 | 1539903 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LukS-PV induces mitochondrial-mediated apoptosis and G0/G1 cell cycle arrest in human acute myeloid leukemia THP-1 cells
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کلمات کلیدی
DDCTFCMPBSAMLCyclinFBScomparative CtLFA-1GAPDHCTCLMTSPMNsSCID3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium - 3- (4،5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazoliumCDKs - CDK هاLymphocyte function-associated antigen-1 - آنتی ژن 1 مرتبط با عملکرد لنفوسیتstandard deviation - انحراف معیارTUNEL - تونلApoptosis - خزان یاختهایfetal bovine serum - سرم جنین گاوTHP-1 cells - سلول های THP-1Phosphate buffered saline - فسفات بافر شورFlow cytometry - فلوسیتومتریCutaneous T-cell lymphoma - لنفوم پوستی Tacute myeloid leukemia - لوسمی حاد میلوئیدی یا به اختصار AMLLeukotoxin - لکوتوکسینpolymorphonuclear leukocytes - لکوسیت های پلی مورفونیک هسته ایMitochondrial pathway - مسیر میتوکندریاییPVL - مقاله از استخراج اجباریPanton-Valentine leukocidin - پانتون-ولنتاین لوکوسیدینPropidium iodide - پروتئین یدیدCell cycle - چرخه سلولیsevere combined immunodeficiency - کمبود شدید مصدومcyclin-dependent kinases - کیناز وابسته به سیکلینglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The S component (LukS-PV) is one of the two components of Panton-Valentine leukocidin (PVL), which is a pore-forming cytotoxin secreted by Staphylococcus aureus, with the ability to lyse leukocytes. In this study, LukS-PV had the ability to induce apoptosis in the human acute myeloid leukemia (AML) cell line THP-1. Therefore, we investigated the mechanisms of LukS-PV-induced apoptosis in THP-1 cells. THP-1 cells treated with LukS-PV, resulted in a significant inhibition of proliferation in a dose- and time-dependent manner, and induced G0/G1 arrest associated with an inhibition of cell cycle arrest regulatory protein (cyclin D1) in a dose- and time-dependent manner, as measured by flow cytometry (FCM). After 12 h exposure to LukS-PV (1.00 μM), annexin V-EGFP/propidium iodide (PI) FCM revealed that 19.5 ± 3.6% of THP-1 cells were apoptotic, and terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) staining also revealed THP-1 cells were apoptotic. Chip analysis of 84 apoptosis-related genes demonstrated that 9 genes were up-regulated at least 2-fold and that 5 genes were down-regulated at least 2-fold in the treatment group when compared with levels in the control group. Western blotting reveled that the expression of caspase-8 increased significantly (approximately 4-fold). The levels of caspase-9, -3 and Bax increased significantly, and levels of Bcl-2 decreased rapidly with LukS-PV treatment. These data suggest that LukS-PV acts as an anti-leukemia agent and activates AML cell apoptosis via the mitochondrial pathway. Therefore, LukS-PV may be a multi-targeting drug candidate for the prevention and therapy of AML.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 8, August 2013, Pages 1531-1537
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 8, August 2013, Pages 1531-1537
نویسندگان
Su Bu, Qiang Xie, Wenjiao Chang, Xingxing Huo, Feihu Chen, Xiaoling Ma,