کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8324519 | 1539912 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prostaglandin E2 stimulates S100A8 expression by activating protein kinase A and CCAAT/enhancer-binding-protein-beta in prostate cancer cells
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کلمات کلیدی
RAGEqRT-PCRERKPGE2pKaCOX-2HRPGSK-3TBSFBSPBSCREBcyclic AMP - AMP cyclicBSA - BSAc/ebpβ - c / ebpβcAMP - cAMPPI 3-kinase - PI 3-کینازS100A8/A9 - S100A8 / A9bovine serum albumin - آلبومین سرم گاوchromatin immunoprecipitation - ایمن سازی کروماتینImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیTris-buffered saline - تریس بافر شورfetal bovine serum - سرم جنین گاوCyclooxygenase-2 - سیکلوکوکسیژناز2Conditioned medium - شرایط محیطیPhosphatidyl Inositol 3-kinase - فسفاتیدیل آنزیم 3-کینازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیhorse radish peroxidase - پراکسیداز تربچه اسبcAMP response element binding protein - پروتئین اتصال دهنده عنصر پاسخ cAMPprotein kinase A - پروتئین کیناز Aprostaglandin E receptor - پروستاگلاندین E receptorProstaglandin E2 - پروستاگلاندین E2prostaglandin - پروستاگلاندینهاCHiP - چیپextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیglycogen synthase kinase-3 - گلیکوزین سنتاز کیناز 3Receptor for advanced glycation end products - گیرنده برای محصولات پیشرفته glycation پایان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Prostaglandin E2 stimulates S100A8 expression by activating protein kinase A and CCAAT/enhancer-binding-protein-beta in prostate cancer cells Prostaglandin E2 stimulates S100A8 expression by activating protein kinase A and CCAAT/enhancer-binding-protein-beta in prostate cancer cells](/preview/png/8324519.png)
چکیده انگلیسی
S100A8 and S100A9 are strongly expressed in epithelial cells of human prostate cancer. However, the regulation of their expression is unclear. Here we show that S100A8 and to a lesser extent S100A9 mRNA expression is induced by prostaglandin E2 in a dose and time-dependent manner in PC-3 prostate cancer cells as well as in BPH-1 benign prostatic epithelial cells. Prostanoid receptor EP2 antagonist AH6809 and EP4 antagonist AH23848, as well as protein kinase A inhibitor H89, inhibited prostaglandin E2 mediated increase in S100A8 mRNA expression as well as promoter activity. Sequence analysis detected a potential binding site of the transcription factor CCAAT/enhancer-binding-protein-beta within the proximal S100A8 promoter. CCAAT/enhancer-binding-protein-beta overexpression increased S100A8 mRNA and protein expression as well as its promoter activity. The latter was prevented by mutation of the potential CCAAT/enhancer-binding-protein-beta binding site within the S100A8 promoter. Chromatin immunoprecipitation revealed increased binding of CCAAT/enhancer-binding-protein-beta to the S100A8 promoter in prostaglandin E2 treated cells. Knockdown of CCAAT/enhancer-binding-protein-beta by siRNA blocked prostaglandin E2 mediated induction of S100A8 promoter activity and mRNA expression. Our results indicate that in prostate cancer cells, S100A8 expression is stimulated by prostaglandin E2 via EP2 and EP4 receptors through activation of the protein kinase A signaling pathway and subsequent stimulation of CCAAT/enhancer-binding-protein-beta binding to the S100A8 promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 44, Issue 11, November 2012, Pages 1919-1928
Journal: The International Journal of Biochemistry & Cell Biology - Volume 44, Issue 11, November 2012, Pages 1919-1928
نویسندگان
Lin Miao, Sina Grebhardt, Jiandang Shi, Isabelle Peipe, Ju Zhang, Doris Mayer,