کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336693 | 1540641 | 2016 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Olive oil compounds inhibit the paracrine regulation of TNF-α-induced endothelial cell migration through reduced glioblastoma cell cyclooxygenase-2 expression
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کلمات کلیدی
COX-2HBMECERKGBMEVOOIκBIL-1βArachidonic acid - اسید آراشیدونیکCNS - دستگاه عصبی مرکزیconditioned media - رسانه های متعلق بهExtra virgin olive oil - روغن زیتون اضافیBBB - سد خونی مغزیBlood–brain barrier - سد خونی مغزیHuman brain microvascular endothelial cells - سلول های اندوتلیال میکرو عروقی مغز انسانcentral nervous system - سیستم عصبی مرکزیCyclooxygenase-2 - سیکلوکوکسیژناز2inhibitor of κB - مهار کننده κBHydroxytyrosol - هیدروکسی تیروسولextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیGlioblastoma multiforme - گلیوبلاستوم مولتیفرم، گلیوبلاستوما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The established causal relationship between the chronic inflammatory microenvironment, tumor development and cancer recurrence has provided leads for developing novel preventive strategies. Accumulating experimental, clinical and epidemiological data has provided support for the chemopreventive properties of olive oil compounds traditionally found within the Mediterranean diet. In this study, we investigated whether tyrosol (Tyr), hydroxytyrosol, oleuropein and oleic acid (OA), four compounds contained in extra virgin olive oil, can prevent tumor necrosis factor (TNF)-α-induced expression of cyclooxygenase (COX)-2 (an inflammation biomarker) in a human glioblastoma cell (U-87 MG) model. We found that Tyr and OA significantly inhibited TNF-α-induced COX-2 gene and protein expression, as well as PGE2 secretion. Both compounds also inhibited TNF-α-induced JNK and ERK phosphorylation, whereas only Tyr inhibited TNF-α-induced NF-κB phosphorylation. Paracrine-regulated migration of human brain microvascular endothelial cells (HBMECs) was assessed using growth factor-enriched conditioned media (CM) isolated from U-87 MG cells. We found that while PGE2 triggered HBMEC migration, the CM isolated from U-87 MG cells, where either COX-2 or NF-κB had been silenced or had been treated with Tyr or OA, exhibited decreased chemotactic properties. These observations demonstrate that olive oil compounds inhibit the effect of the chronic inflammatory microenvironment on glioblastoma progression through TNF-α actions and may be useful in cancer chemoprevention.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 27, January 2016, Pages 136-145
Journal: The Journal of Nutritional Biochemistry - Volume 27, January 2016, Pages 136-145
نویسندگان
Sylvie Lamy, Aroua Ben Saad, Alain Zgheib, Borhane Annabi,