کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8337789 | 1540966 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation and function of runt-related transcription factors (RUNX1 and RUNX2) in goat granulosa cells
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کلمات کلیدی
PKCRunx13β-Hydroxysteroid dehydrogenaseCYP11A1phorbol 12-myristate 13-acetatePtgs2CYP19A1forskolinTNFAIP6Hsd3bpKaFskHapln1RUNX2PI3K3′ UTR - 3 UTR3′ untranslated regions - 3 منطقه غیر ترجمهPMA - LDC هاSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAHCG - اچ سی جیGoat - بزStar - ستارهgranulosa cell - سلول گرانولوزاRunt-related transcription factor 2 - عامل رونویسی مرتبط با روت 2runt-related transcription factor 1 - عامل رونویسی مرتبط با ریت 1phosphatidylinositol 3 kinase - فسفاتیدیلینوزیتول 3 کینازluteinizing hormone - هورمون جسم زردSteroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سیprostaglandin-endoperoxide synthase 2 - پروستاگلاندین اندپورکسید سنتاز 2human chorionic gonadotropin - گونادوتروپین کوریونی انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Transcription factors, runt-related transcription factor 1 (RUNX1) and 2 (RUNX2), belong to the runt-related (RUNX) gene family and play critical roles in mammalian reproduction processes. However, the regulatory mechanisms of RUNX1 and RUNX2 expression or their functions in goat follicles remain largely unknown. Herein, RUNX1 and RUNX2 proteins were detected in the oocytes and granulosa cells of preantral and antral follicles, as well as corpus luteum by immunohistochemistry. Treatments with human chorionic gonadotropin (hCG) or with the agonists and inhibitors of hCG-induced intracellular signaling pathways in granulosa cells in vitro, we found that hCG increased RUNX1 expression by activating PKC and PI3K signaling molecules, and increased RUNX2 expression by activating adenylate cyclase, PKC, and PI3K signaling molecules. We also demonstrated that miR-181b expression is dependent on the hCG-induced activation of PKC and PKA, and miR-222 expression is dependent on the hCG-induced activation of PI3K and PKC in cultured granulosa cells. Meanwhile, miR-181b and miR-222 suppressed RUNX1 and RUNX2 expression by targeting RUNX1 and RUNX2 3â² untranslated regions (3â²UTRs) with or without hCG, respectively. These results suggested that hCG-mediated miR-181b and miR-222 expression are important for the regulation of RUNX1 and RUNX2 expression levels in granulosa cells. To explore the specific functions of RUNX1 and RUNX2, we transfected RUNX1 and RUNX2 small interfering RNAs into primary cultured granulosa cells. Knockdown of RUNX1 and RUNX2 significantly decreased progesterone productions and the mRNA abundance of key steroidogenic enzymes (StAR, CYP11A1 and HSD3B) after hCG treatment. But only miR-222 increased estradiol secretion in goat granulosa cells. In addition, knockdown of RUNX1 and RUNX2 also promoted granulosa cell proliferation. The hormonally regulated expression of RUNX1 and RUNX2 in granulosa cells, their involvement in progesterone production, and promoted granulosa cell proliferation suggest important roles of RUNX1 and RUNX2 in follicular development and luteinization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 181, July 2018, Pages 98-108
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 181, July 2018, Pages 98-108
نویسندگان
Kexin Gao, Peijie Wang, Jiayin Peng, Junjun Xue, Kaiwen Chen, Yuxuan Song, Jiangang Wang, Guang Li, Xiaopeng An, Binyun Cao,