کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8339620 | 1541029 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endocrine disrupting activity in fruits and vegetables evaluated with the E-screen assay in relation to pesticide residues
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کلمات کلیدی
DMEMProliferative effect17b-estradiolEEQeefRPEFCSEFSAEC50SPEDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoEDCs - EDC هاGC–MS - GC-MSLIG - LIGApesticides - آفتکشهاEuropean Food Safety Authority - اداره ایمنی اروپاEuropean - اروپاییSolid phase extraction - استخراج فاز جامدISO - ایزوIsoflavones - ایزوفلاونهاTAM - تامtamoxifen - تاموکسیفنendocrine disruptor compounds - ترکیبات نارسایی اندوکرینWorld Health Organization - سازمان بهداشت جهانیVegetables - سبزیجاتfetal calf serum - سرم گوساله جنینE-screen - صفحه نمایش الکترونیکیgas chromatography–mass spectrometry - طیف سنجی جرم کروماتوگرافی گازeffective concentration 50 - غلظت موثر 50Estrogenic activity - فعالیت استروژنیکLignans - لیگنانSpearman rank correlation - همبستگی رتبه اسپیرمنHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراWHO - کهCoumestrol - کومسترولCou - کوچه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Food is likely to be one of the most important routes of human exposure to endocrine disrupting compounds (EDCs). In the present study, we evaluated the total estrogenic activity of fruits and vegetables, which was calculated using the human breast cancer cell line (MCF-7 BUS) proliferation assay (E-screen), in relation to pesticide residues. We analysed 44 food samples, 30 fruits and 14 vegetables. Of these samples, 10 did not contain any pesticide residues. The other 34 samples contained from 1 to 7 pesticide residues in concentrations ranging from 0.03 to 1.91 ppm. Estrogenic activity was detected in the 59% of samples tested. The positive controls used were 17-β-estradiol (E2), the phytoestrogen genistein and the pesticide endosulfan. The average value of estradiol equivalency quantity (EEQ) for all positive samples was 0.15 ± 0.32 μg/100 g. A low correlation was found between the concentration of pesticide residues and the EEQ values (Spearman correlation r = 0.376 and p = 0.012). Using values obtained from the literature, we compared the estrogenic activity of food samples with the intrinsic content of phytoestrogens, but we found no correlations. Our results also suggested that the calculated intake of dietary EDCs might represent a concentration comparable to the normal endogenous estrogen concentration in human blood.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 127, Issues 1â2, October 2011, Pages 139-146
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 127, Issues 1â2, October 2011, Pages 139-146
نویسندگان
T. Schilirò, I. Gorrasi, A. Longo, S. Coluccia, G. Gilli,