کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8345200 | 1541613 | 2015 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The reaction products of sulfide and S-nitrosoglutathione are potent vasorelaxants
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کلمات کلیدی
ODQMGDSGCABSS-nitrosoglutathioneNACGSNON-acetyl-l-cysteine1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one - 1H- [1،2،4] اکسیدیاازولو [4،3-a] کینوکسالین-1-یون2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide - 2- (4-کاربوکسی فنیل) -4،4،5،5-تترامتییلیمیدازولین-1-اکسیل -3 اکسیدAUC - AUCcPTIO - eptirElectron paramagnetic resonance - تشدید پارامغناطیس الکترونEPR - تشدید پارامغناطیس الکترونAbsorbance - جذبSoluble guanylyl cyclase - حلال گویینیل سیکلاسPhen - فینarea under the curve - منطقه تحت منحنیAngeli's salt - نمک آنجلیNitroprusside - نیتروپروسیدPolysulfides - پلی سولفیدSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The chemical interaction of sodium sulfide (Na2S) with the NO-donor S-nitrosoglutathione (GSNO) has been described to generate new reaction products, including polysulfides and nitrosopersulfide (SSNO-) via intermediacy of thionitrous acid (HSNO). The aim of the present work was to investigate the vascular effects of the longer-lived products of the Sulfide/GSNO interaction. Here we show that the products of this reaction relax precontracted isolated rings of rat thoracic aorta and mesenteric artery (but to a lesser degree rat uterus) with a >2-fold potency compared with the starting material, GSNO (50ânM), whereas Na2S and polysulfides have little effect at 1-5âµM. The onset of vasorelaxation of the reaction products was 7-10 times faster in aorta and mesenteric arteries compared with GSNO. Relaxation to GSNO (100-500ânM) was blocked by an inhibitor of soluble guanylyl cyclase, ODQ (0.1 and 10âµM), and by the NO scavenger cPTIO (100âµM), but less affected by prior acidification (pH 2-4), and unaffected by N-acetylcysteine (1âmM) or methemoglobin (20âµM heme). By contrast, relaxation to the Sulfide/GSNO reaction products (100-500ânM based on the starting material) was inhibited to a lesser extent by ODQ, only slightly decreased by cPTIO, more markedly inhibited by methemoglobin and N-acetylcysteine, and abolished by acidification before addition to the organ bath. The reaction mixture was found to generate NO as detected by EPR spectroscopy using N-(dithiocarboxy)-N-methyl-D-glucamine (MGD2)-Fe2+ as spin trap. In conclusion, the Sufide/GSNO reaction products are faster and more pronounced vasorelaxants than GSNO itself. We conclude that in addition to NO formation from SSNO-, reaction products other than polysulfides may give rise to nitroxyl (HNO) and be involved in the pronounced relaxation induced by the Sulfide/GSNO cross-talk.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 46, 30 April 2015, Pages 123-130
Journal: Nitric Oxide - Volume 46, 30 April 2015, Pages 123-130
نویسندگان
Andrea Berenyiova, Marian Grman, Ana Mijuskovic, Andrej Stasko, Anton Misak, Peter Nagy, Elena Ondriasova, Sona Cacanyiova, Vlasta Brezova, Martin Feelisch, Karol Ondrias,