کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8355237 | 1541949 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential expression of flavonoid 3â²-hydroxylase during fruit development establishes the different B-ring hydroxylation patterns of flavonoids in Fragaria à ananassa and Fragaria vesca
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Flavonoid 3â²-hydroxylase (F3â²H) was studied for the first time in different Fragaria species. The cDNA clones isolated from unripe and ripe fruits of Fragaria x ananassa (garden strawberry) and Fragaria vesca (wild strawberry) showed high similarity (99% at the amino acid level) to the publically available F. vesca genome sequence and no significant differences could be identified between species and developmental stages of the fruits. In contrast, the genomic F3â²H clones showed differences in the non-coding regions and 5â²-flanking elements. The recombinant F3â²Hs were functionally active and showed high specificity for naringenin, dihydrokaempferol, and kaempferol, whereas apigenin was only a minor substrate. During fruit development, a clear difference in the F3â²H expression was observed between F. à ananassa and F. vesca. While a drastic decline of F3â²H expression occurred during fruit ripening in F. à ananassa, F3â²H in F. vesca was highly expressed in all stages. This was reflected by the anthocyanin composition, which showed a prevalence of pelargonidin in ripe fruits of F. à ananassa, whereas F. vesca had a high content of cyanidin. Screening of 17 berry species for their anthocyanin and flavonol composition showed that the prevalence of monohydroxylated anthocyanins makes garden strawberry unique among all other fruit species indicating that selection of bright red color during strawberry breeding, which consumers typically associate with freshness and ripeness, has selected phenotypes with a special biochemical background.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 72, November 2013, Pages 72-78
Journal: Plant Physiology and Biochemistry - Volume 72, November 2013, Pages 72-78
نویسندگان
Jana Thill, Silvija Miosic, Tek Prasad Gotame, Maja Mikulic-Petkovsek, Christian Gosch, Robert Veberic, Anja Preuss, Wilfried Schwab, Franci Stampar, Karl Stich, Heidi Halbwirth,