کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8355518 | 1541951 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots
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کلمات کلیدی
2-DEISBPeptide mass finger printingSLMMethyl jasmonate (MeJA)IEFCBBAPXPMFIgGPODCoomassie Brilliant Blue - Coomassie درخشان آبیMALDI-TOF/TOF - MALDI-TOF / TOFtaxifolin - taxfilinjasmonic acid - اسید جاسونیکtwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدLOX - اکسیژن مایعisoelectric focusing - تمرکز ذره ای الکتریکیHairy root culture - ریشه کثیف ریشهSilydianin - سیلدیانینSilymarin - سیلیمارین Mass spectrometry - طیف سنجی جرمیlipoxygenase - لیپواکسیژنازFAME یا fatty acid methyl esters - متیل استرهای اسید چربfatty acid methyl esters - متیل استرهای اسید چربMethyl jasmonate - متیل جاسموناتMeJA - میاPeroxidase - پراکسیدازascorbate peroxidase - پراکسیداز آسکورباتیhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراGas chromatography - کروماتوگرافی گازی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots](/preview/png/8355518.png)
چکیده انگلیسی
Jasmonate and its methyl derivative, methyl jasmonate (MeJA), are naturally occurring compounds that mediate several plant physiological processes in response to pathogen attack, wounding, and ozone. Exogenous application of jasmonates triggers defense responses that resemble those initiated by pathogen infection and also modulates the production of certain secondary metabolites in a variety of plant species. In this study, we treated the hairy root cultures of Silybum marianum L. with 100 μM MeJA and then measured the content of Silymarin (SLM). We observed that the SLM content increased significantly after 48 h of MeJA treatment and remained constant for 120 h. However, MeJA treatment caused a significant growth reduction after 96 h incubation. The activity of lipoxygenase as a key enzyme in the jasmonate biosynthesis pathway and anti-oxidative enzymes; peroxidase and ascorbate peroxidase was also significantly increased after MeJA treatment. To elucidate the global effect of jasmonate on gene expression of S. marianum, we employed high resolution two-dimensional gel electrophoresis coupled with tandem mass spectrometry. Out of 670 reproducibly detected protein spots which were analyzed on each given gel, 32 spots were up- or down regulated upon MeJA treatment. Of them, ten proteins such as ER binding protein, glutamine synthetase, pathogenesis-related protein, caffeoyl CoA O-methyltransferase, and profilin-1 could be identified by mass spectrometry analysis. The possible implications of the identified proteins on physiological outcome of MeJA application in S. marianum hairy root culture will be discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 70, September 2013, Pages 115-122
Journal: Plant Physiology and Biochemistry - Volume 70, September 2013, Pages 115-122
نویسندگان
Javad Gharechahi, Masumeh Khalili, Tahereh Hasanloo, Ghasem Hosseini Salekdeh,