کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357314 | 1542047 | 2016 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes
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کلمات کلیدی
MABCMarker-assisted backcrossingHDLAS-PCRCAPSILS - آنهاOleic acid - اسید اولئیکFatty acid desaturase - اسید لاکتیک اسیدmarker-assisted selection - انتخاب کمک کننده نشانگرPeanut - بادام زمینی، پستهشامیFAD - بدMAS - بیشترCardiovascular diseases - بیماری قلبی-عروقی Introgression lines - خطوط درونیCVD - رسوب دهی شیمیایی بخار low density lipoproteins - لیپوپروتئین های چگالی کمLDL - لیپوپروتئین کم چگالی(کلسترول بد)high density lipoproteins - لیپوپروتئینهای چگالی بالاOil content - محتوای نفتallele specific polymerase chain reaction - واکنش زنجیره ای پلی مراز خاص آللOil quality - کیفیت روغن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes](/preview/png/8357314.png)
چکیده انگلیسی
High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 (ahFAD2A) and b09 (ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142 and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5-1.1 folds, with concomitant reduction of linoleic acid by 0.4-1.0 folds and palmitic acid by 0.1-0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53-58%), and 28 ILs with low oil content (42-50%) were selected that may be released for cultivation upon further evaluation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 242, January 2016, Pages 203-213
Journal: Plant Science - Volume 242, January 2016, Pages 203-213
نویسندگان
Pasupuleti Janila, Manish K Pandey, Yaduru Shasidhar, Murali T Variath, Manda Sriswathi, Pawan Khera, Surendra S Manohar, Patne Nagesh, Manish K Vishwakarma, Gyan P Mishra, T Radhakrishnan, N Manivannan, KL Dobariya, RP Vasanthi, Rajeev K Varshney,