کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357823 | 1542055 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dissecting root proteome of transgenic rice cultivars unravels metabolic alterations and accumulation of novel stress responsive proteins under drought stress
ترجمه فارسی عنوان
ردپای پروتئین ریشه ای از ارقام برنجی ترانس ژنیک، تغییرات متابولیک و انباشت پروتئین های پاسخ دهنده استرس را تحت تنش خشکی
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کلمات کلیدی
ABASnRK2DREB2DE - 2deIPG - GPAimmobilized pH gradient - gradient pH immobilizedROS - ROSabscisic acid - اسید آبسزیکtwo dimensional gel electrophoresis - الکتروفورز ژل دو بعدیtwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیGDP - تولید ناخالص ملیDrought - خشکسالیRoot - ریشهMatrix assisted laser desorption ionization-time of flight mass spectrometry - ماتریکس به زمان یونیزاسیون لیزر جذب طیف سنجی جرمی کمک کردMALDI-TOF MS - مالدی توف MSwild type - نوع وحشیProteomics - پروتئومیکسguanosine diphosphate - گوانوزین دی فسفاتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Generation of drought tolerant rice plants by overexpressing Arabidopsis DREB1A is a significant development for abiotic stress research. However, the metabolic network regulated in the drought tolerant transgenic rice plants is poorly understood. In this research study, we have demonstrated the comparative proteome analysis between the roots of wild type and transgenic DREB1A overexpressing homozygous plants under drought stress condition. After 7Â d of dehydration stress at reproductive stage, the plants were re-watered for 24Â h. The roots were collected separately from wild type and transgenic plants grown under water, drought stress and re-watering conditions and total proteins were analyzed by two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS). Among the large number of differentially accumulated spots, 30, 27 and 20 spots were successfully identified as differentially expressed proteins in three different conditions respectively. The major class of identified proteins belongs to carbohydrate and energy metabolism category while stress and defense related proteins are especially up-accumulated under drought stress in both the plants. A novel protein, R40C1 was reported to be up-accumulated in roots of transgenic plants which may play an important role in generation of drought tolerant plants. Protein-protein interaction helps to identify the network of drought stress signaling pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 234, May 2015, Pages 133-143
Journal: Plant Science - Volume 234, May 2015, Pages 133-143
نویسندگان
Soumitra Paul, Dipak Gayen, Swapan K. Datta, Karabi Datta,