کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8417414 | 1545685 | 2015 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Methods to monitor monocytes-mediated amyloid-beta uptake and phagocytosis in the context of adjuvanted immunotherapies
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کلمات کلیدی
Aβ uptakeTLREOADMPLAPPRPMIPresenilin-1HRPGMFIAβDPBSgeometric mean of fluorescence intensityPiBFMOsPS-1late-onset ADDMEMLPSPS-2PBSEarly-onset ADBSA - BSADulbecco's modified Eagle Medium - Eagle Medium اصلاح شده Dulbeccopresenilin-2 - Presenilin-2ROS - ROSbovine serum albumin - آلبومین سرم گاوamyloid-beta - آمیلوئید بتاinsulin-degrading enzyme - آنزیم تحقیر کننده انسولینAdjuvant - ادجوانت، یاورIDE - اینجاLoad - بارAlzheimer's disease - بیماری آلزایمرToll-like receptor - تیالآرintra-venous - داخل وریدیintra-muscular - درون عضلانیfigure - شکلFig. - شکل.Phagocytosis - فاگوسیتوز یا بیگانهخواری Dulbecco's phosphate buffered saline - فسفات باسیل نمک Dulbeccofluorescence minus one - فلورسانس منهای یکlipopolysaccharide - لیپوپلی ساکاریدMonophosphoryl lipid A - لیپید مونوفسفریل APhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریRoswell Park Memorial Institute medium - موسسه خاطرات Roswell Park mediumMonocyte - مونوسیتVaccine - واکسنHorseradish peroxidase - پراکسیداز هوررادیشamyloid precursor protein - پروتئین پیش ماده آمیلوئیPittsburgh compound B - پیتسبورگ ترکیب BReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Methods to monitor monocytes-mediated amyloid-beta uptake and phagocytosis in the context of adjuvanted immunotherapies Methods to monitor monocytes-mediated amyloid-beta uptake and phagocytosis in the context of adjuvanted immunotherapies](/preview/png/8417414.png)
چکیده انگلیسی
Antibody-mediated capture of amyloid-beta (Aβ) in peripheral blood was identified as an attractive strategy to eliminate cerebral toxic amyloid in Alzheimer's disease (AD) patients and murine models. Alternatively, defective capacity of peripheral monocytes to engulf Aβ was reported in individuals with AD. In this report, we developed different approaches to investigate cellular uptake and phagocytosis of Aβ, and to examine how two immunological devices - an immunostimulatory Adjuvant System and different amyloid specific antibodies - may affect these biological events. Between one and thirteen months of age, APPswe X PS1.M146V (TASTPM) AD model mice had decreasing concentrations of Aβ in their plasma. In contrast, the proportion of blood monocytes containing Aβ tended to increase with age. Importantly, the TLR-agonist containing Adjuvant System AS01B primed monocytes to promote de novo Aβ uptake capacity, particularly in the presence of anti-Aβ antibodies. Biochemical experiments demonstrated that cells achieved Aβ uptake and internalization followed by Aβ degradation via mechanisms that required effective actin polymerization and proteolytic enzymes such as insulin-degrading enzyme. We further demonstrated that both Aβ-specific monoclonal antibodies and plasma from Aβ-immunized mice enhanced the phagocytosis of 1 μm Aβ-coated particles. Together, our data highlight a new biomarker testing to follow amyloid clearance within the blood and a mechanism of Aβ uptake by peripheral monocytes in the context of active or passive immunization, and emphasize on novel approaches to investigate this phenomenon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 424, September 2015, Pages 64-79
Journal: Journal of Immunological Methods - Volume 424, September 2015, Pages 64-79
نویسندگان
Maxime Hallé, Pascale Tribout-Jover, Anne-Marie Lanteigne, Jonathan Boulais, Julien R. St-Jean, Rachel Jodoin, Marie-Pier Girouard, Florin Constantin, Annik Migneault, Frédéric Renaud, Arnaud M. Didierlaurent, Corey P. Mallett, David Burkhart,