کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456754 | 1548772 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Telomeric Recombination Induced by DNA Damage Results in Telomere Extension and Length Heterogeneity
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کلمات کلیدی
DOXTRFCO-FISHDDRPMLALTTRAPDSBsAPBSRTLBIR - BRstandard deviation - انحراف معیارbreak-induced replication - تکرار ناشی از شکستگیdoxycycline - داکسی سایکلینdouble-strand breaks - شکست دو ردیفRelative telomere length - طول تلومر نسبیAlternative lengthening of telomeres - طولانی شدن جایگزین تلومرهاtelomere restriction fragment - قطعه محدود کننده تلومرPromyelocytic leukemia - لوسمی PromyelocyticHomologous recombination - نوترکیبی همولوگDNA damage response - واکنش به آسیب DNA Telomeric repeat amplification protocol - پروتکل تقویت تکرار تلومر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
About 15% of human cancers counteract telomere loss by alternative lengthening of telomeres (ALT), which is attributed to homologous recombination (HR)-mediated events. But how telomeric HR leads to length elongation is poorly understood. Here, we explore telomere clustering and telomeric HR induced by double-stranded breaks (DSBs). We show that telomere clustering could occur at G1 and S phase of cell cycle and that three types of telomeric HR occur based on the manner of telomeric DNA exchange: equivalent telomeric sister chromatin exchange (T-SCE), inequivalent T-SCE, and No-SCE. While inequivalent T-SCE increases telomere length heterogeneity with no net gain of telomere length, No-SCE, which is presumably induced by interchromatid HR and/or break-induced replication, results in telomere elongation. Accordingly, cells subjected to long-term telomeric DSBs display increased heterogeneity of length and longer telomeres. We also demonstrate that DSBs-induced telomere elongation is telomerase independent. Moreover, telomeric recombination induced by DSBs is associated with formation of ALT-associated PML body and C-circle. Thus, DNA damage triggers recombination mediated elongation, leading to the induction of multiple ALT phenotypes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neoplasia - Volume 20, Issue 9, September 2018, Pages 905-916
Journal: Neoplasia - Volume 20, Issue 9, September 2018, Pages 905-916
نویسندگان
Haiying Liu, Yujie Xie, Zepeng Zhang, Pingsu Mao, Jingfan Liu, Wenbin Ma, Yong Zhao,