کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8473088 | 1550383 | 2018 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Temperature-sensitive sarcomeric protein post-translational modifications revealed by top-down proteomics
ترجمه فارسی عنوان
پروتئومیک های بالا از پایین پروتئین های پروتئومیک پایین تر از پروتئین های سارکوبریک حساس به دما دارند
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کلمات کلیدی
Fourier transform-ion cyclotron resonanceCK2cTnIcTNTPKGELCcMyBP-cRLCpKaTPMpNPPPTMsQ-TOFTFApara-nitrophenylphosphatePP2AFT-ICRECDSarcomerescAMP - cAMPMS/MS - MS / MSRPc - RPCCyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییTrifluoroacetic acid - اسید Trifluoroaceticpost-translational modification - اصلاح post-translationalTandem MS - تاندوم MSTropomyosin - تروپومیوسینcardiac troponin T - تروپونین T قلبELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاpost-translational modifications - تغییرات پس از ترجمه، پیرایش پساترجمهElectron capture dissociation - جداسازی الکترونیquadrupole time-of-flight - زمان پرواز چهار روزهessential light chain - زنجیره ای ضروری ضروری استregulatory light chain - زنجیره سبک قانونیTop-down mass spectrometry - طیف سنج جرمی بالاMass spectrometry - طیف سنجی جرمیPhosphorylation - فسفریلاسیونcardiac troponin I - قلب تروپونین Iheart failure - نارسایی قلبیcardiac myosin binding protein C - پروتئین C اتصال مایوسین قلبیprotein phosphatase 2A - پروتئین فسفاتاز 2Aprotein kinase A - پروتئین کیناز Aprotein kinase G - پروتئین کیناز GCasein kinase 2 - کازئین کیناز 2reverse phase chromatography - کروماتوگرافی فاز معکوسliquid chromatography - کروماتوگرافی مایعHigh pressure liquid chromatography - کروماتوگرافی مایع با فشار بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
Despite advancements in symptom management for heart failure (HF), this devastating clinical syndrome remains the leading cause of death in the developed world. Studies using animal models have greatly advanced our understanding of the molecular mechanisms underlying HF; however, differences in cardiac physiology and the manifestation of HF between animals, particularly rodents, and humans necessitates the direct interrogation of human heart tissue samples. Nevertheless, an ever-present concern when examining human heart tissue samples is the potential for artefactual changes related to temperature changes during tissue shipment or sample processing. Herein, we examined the effects of temperature on the post-translational modifications (PTMs) of sarcomeric proteins, the proteins responsible for muscle contraction, under conditions mimicking those that might occur during tissue shipment or sample processing. Using a powerful top-down proteomics method, we found that sarcomeric protein PTMs were differentially affected by temperature. Specifically, cardiac troponin I and enigma homolog isoform 2 showed robust increases in phosphorylation when tissue was incubated at either 4â¯Â°C or 22â¯Â°C. The observed increase is likely due to increased cyclic AMP levels and activation of protein kinase A in the tissue. On the contrary, cardiac troponin T and myosin regulatory light chain phosphorylation decreased when tissue was incubated at 4â¯Â°C or 22â¯Â°C. Furthermore, significant protein degradation was also observed after incubation at 4â¯Â°C or 22â¯Â°C. Overall, these results indicate that temperature exerts various effects on sarcomeric protein PTMs and careful tissue handling is critical for studies involving human heart samples. Moreover, these findings highlight the power of top-down proteomics for examining the integrity of cardiac tissue samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 122, September 2018, Pages 11-22
Journal: Journal of Molecular and Cellular Cardiology - Volume 122, September 2018, Pages 11-22
نویسندگان
Wenxuan Cai, Zachary L. Hite, Beini Lyu, Zhijie Wu, Ziqing Lin, Zachery R. Gregorich, Andrew E. Messer, Sean J. McIlwain, Steve B. Marston, Takushi Kohmoto, Ying Ge,