کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8474361 | 1550423 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation
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کلمات کلیدی
VSMCUbc9SUMOylationKLFHASMCVSMCsCDK2LSD1HDAC2KLF4PDGFP21NCoRSRFSUMOMIRqRT-PCRatRA - ATRAp21WAF1/Cip1 - p21WAF1 / Cip1all-trans retinoic acid - آل – ترانس رتینوئیک اسیدlysine-specific demethylase 1 - الکل دیمیتیلاز 1miR-200c - به miR-200CProliferation - ترویجhuman aortic smooth muscle cell - سلول عضله صاف آئورت انسانVascular smooth muscle cells - سلول های عضلانی صاف عروقیcyclin-dependent kinase 2 - سیکلین وابسته به کیناز 2Krüppel-like factor - عامل کریپال مانندplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتserum response factor - فاکتور پاسخ سرمیUntranslated regions - مناطق غیر ترجمهUTR یا untranslated regions - منطقه ترجمه نشدهMicroRNA - میکرو RNA nuclear receptor corepressor - هسته گیرنده گیرنده اتمیHistone deacetylase 2 - هیستون دیازتیلاز 2quantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استsmall ubiquitin-like modifier - کوچک مانند ubiquitin مانند اصلاح کننده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation](/preview/png/8474361.png)
چکیده انگلیسی
The regulation of vascular smooth muscle cell (VSMC) proliferation is an important issue because it has major implications for the prevention of pathological vascular conditions. Using microRNA array screen, we found the expression levels of 200 unique miRNAs in hyperplasic tissues. Among them, miR-200c expression substantially was down-regulated. The objective of this work was to assess the function of miR-200c and SUMOylated KrÏppel-like transcription factor 4 (KLF4) in the regulation of VSMC proliferation in both cultured cells and animal models of balloon injury. Under basal conditions, we found that miR-200c inhibited the expression of KLF4 and the SUMO-conjugating enzyme Ubc9. Upon PDGF-BB treatment, Ubc9 interacted with and promoted the SUMOylation of KLF4, which allowed the recruitment of transcriptional corepressors (e.g., nuclear receptor corepressor (NCoR) and HDAC2) to the miR-200c promoter. The reduction in miR-200c levels led to increased target gene expression (e.g., Ubc9 and KLF4), which further repressed miR-200c levels and accelerated VSMC proliferation. These results demonstrate that induction of a miR-200c-SUMOylated KLF4 feedback loop is a significant aspect of the PDGF-BB proliferative response in VSMCs and that targeting Ubc9 represents a novel approach for the prevention of restenosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 82, May 2015, Pages 201-212
Journal: Journal of Molecular and Cellular Cardiology - Volume 82, May 2015, Pages 201-212
نویسندگان
Bin Zheng, Michel Bernier, Xin-hua Zhang, Toru Suzuki, Chan-quan Nie, Yong hui Li, Yong Zhang, Li-li Song, Hui-jing Shi, Yan Liu, Cui-ying Zheng, Jin-kun Wen,