کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8485693 | 1551747 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inactivated influenza vaccine stress can affect in vitro potency assay relationship to immunogenicity
ترجمه فارسی عنوان
استرس واکسن آنفولانزای غیر فعال شده می تواند رابطه آزمایش اکتشافی درونمایه با ایمنی بدن را تحت تاثیر قرار دهد
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
SRIDRP-HPLCRBDMDCKIDMSIIV - 7AUC - AUCInfluenza - آنفلوانزا Stress - استرس یا فشار روانیImmunogenicity - ایمنی زاییTrypsinization - تریپسینیزاسیونPotency - تواناییReceptor binding domain - دامنه گیرنده گیرندهIsotope dilution mass spectrometry - طیف سنجی جرمی رقیق ایزوتوپhemagglutination inhibition - مهار هموگلوبینmicroneutralization - میکروسکوپ الکترونیarea-under-curve - ناحیه زیر منحنیhemagglutinin - هماگلوتینینVaccine - واکسنInactivated influenza vaccine - واکسن آنفلوآنزا غیر فعال شده استreversed-phase high pressure liquid chromatography - کروماتوگرافی مایع با فشار فاز معکوسMadin-Darby canine kidney - کلیه های سگ کوچولو Madin-Darby
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Influenza vaccines are the most effective intervention to prevent the substantial public health burden of seasonal and pandemic influenza. The capability of hemagglutinin (HA), the main antigen in inactivated influenza vaccines (IIVs), to elicit functional neutralizing antibodies determines IIV effectiveness. When HA is subjected to environmental stress during manufacturing or while stored prior to administration, such as low pH and temperature excursions, the HA immunological activity can be affected. Single-radial immunodiffusion (SRID), the standard in vitro potency assay for IIVs, is believed to specifically detect immunologically active HA and has been applied to evaluate HA stability against stress. Here we report that transient low pH treatment and freeze/thaw cycles with HA in PBS abolish SRID-quantified in vitro potency for all HAs of multiple influenza strains. Raised temperature substantially decreases in vitro potency with more extensive HA structural changes. Chemical stress and mechanical stress moderately change SRID in vitro potency values in a strain-dependent manner. Trypsin digestion, which selectively degrades stressed HA, followed by RP-HPLC quantification as a candidate alternative in vitro potency assay yields results comparable to SRID. Mouse immunogenicity studies confirm that HA stressed by transient low pH treatment does not elicit functional antibodies in vivo, nor does it have a measureable SRID value. However, HA stressed by raised temperature elicits high titers of functional antibodies in vivo despite substantial loss of SRID in vitro potency. This discrepancy between SRID in vitro potency and vaccine immunogenicity suggests that SRID may not reliably indicate IIV potency under all conditions. Further efforts to develop alternate potency assays that can better predict in vivo immunogenicity should continue along with additional studies exploring HA conformation, SRID values and consequent immunogenicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 36, Issue 21, 17 May 2018, Pages 3010-3017
Journal: Vaccine - Volume 36, Issue 21, 17 May 2018, Pages 3010-3017
نویسندگان
Yingxia Wen, Giuseppe Palladino, Yuhong Xie, Annette Ferrari, Ethan C. Settembre,