کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8507171 | 1555961 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic profiling of the midgut contents of Haemaphysalis flava
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
SDSHaemaphysalis flavaLFQFASPVitellogeninHCDBCAACNDTTFDRNGSLC–MS/MS - LC-MS / MSLabel free quantification - آزمایشی رایگانAcetonitrile - استونیتریلSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتhigher-energy collisional dissociation - انحلال تصادفی بالقوه انرژیbicinchoninic acid - بیسینکنینیک اسیدNext generation sequencing - توالی نسل بعدیsodium dodecyl sulfate - سدیم دودسیل سولفاتliquid chromatography tandem-mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایعfalse discovery rate - میزان کشف کاذبBlood meal - وعده غذاییProteome - پروتئومDigestion - گوارش یا هضم یا تحلیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Scant information is available regarding the proteins involved in blood meal processing in ticks. Here, we aimed to highlight the midgut proteins involved in preventing blood meal coagulation, and in facilitating intracellular digestion in the tick Haemaphysalis flava. Proteins were extracted from the midgut contents of fully engorged and partially engorged ticks. We used liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify 131 unique peptides, and 102 proteins. Of these, 15 proteins, each with at least two unique peptides, were recognized with high confidence. We also retrieved 18 unigenes from our previous published transcriptomic libraries of the midguts and salivary glands of H. flava, and inferred the primary structures of nine proteins and fragments of five proteins. There were 23 and 21 unique proteins in the midgut contents of fully engorged and partially engorged ticks, respectively. We detected 58 shared proteins in the midgut contents of both fully engorged and partially engorged ticks. Of these, seven were significantly differentially expressed between fully engorged and partially engorged ticks: actin, calmodulin, elongation factor-1α, hsp90, multifunctional chaperone, tubulin α, and tubulin β. Our results demonstrated that the proteome of the midgut contents, combined with the transcriptome of the midgut, was a viable method for the reinforcement of protein identification. This method will facilitate further study of blood meal processing by ticks, as well as the identification of clues for tick infestation control. The existence of numerous proteins detected in the midgut contents also highlight the complexity of blood digestion in ticks; this area is in need of further investigation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 3, March 2018, Pages 490-495
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 3, March 2018, Pages 490-495
نویسندگان
Lei Liu, Tian-yin Cheng, Xiao-ming He,