کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8516958 | 1556584 | 2018 | 55 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Orexin-B modulates synaptic transmission of rod bipolar cells in rat retina
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کلمات کلیدی
VTAIPLPKCPTXSCNDT-PBSOPLPLCIPSCsBicucullinePKGDLGphorbol 12-myristate 13-acetateRBCsOX1RpKaPI-PLCOX2RERGTPMPAl-AP4PC-PLCBICCPPGPBSPhosphatidylinositol-specific phospholipase C - Fosfatidylinositol خاص فسفولیپاز Cl-(+)-2-amino-4-phosphonobutyric acid - L - (+) - 2-amino-4-phosphonobutyric acidPMA - LDC هاStrychnine - استریچنینیOrexin - اورکسینphosphate buffer - بافر فسفاتinhibitory postsynaptic currents - جریانهای پسینپتیک مهارکنندهdiacylglycerol - دیسیل گلیسیرینDAG - روزBCs - روند BCsBipolar cells - سلولهای دو قطبیpertussis toxin - سموم سورافنیPhosphate buffered saline - فسفات بافر شورphosphatidylinositol - فسفاتیدیل اینوزیتولphosphatidylcholine - فسفاتیدیل کولینPhosphatidylcholine-specific phospholipase C - فسفاتیدیل کولین خاص فسفولیپاز Cphospholipase C - فسفولیپاز Couter plexiform layer - لایه بیرونی بیرونیinner plexiform layer - لایه داخلی لایهventral tegmental area - ناحیه تگمنتوم شکمیNeuromodulation - نورومدولاسیون dorsal lateral geniculate nucleus - هسته منشاء جانبی پشتی پشتیSuprachiasmatic nuclei - هسته های Suprachiasmaticprotein kinase A - پروتئین کیناز Aprotein kinase G - پروتئین کیناز GProtein kinase C - پروتئین کیناز سیGABAAR - گابارGABA receptor - گیرنده GABAGABAA receptor - گیرنده GABAA یا گیرنده گاباGABAC receptor - گیرنده GABACOrexin receptor 2 - گیرنده اورکسین 2orexin receptor 1 - گیرنده اکسین 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Orexin-A, -B play a crucial role in arousal and feeding by activating two G-protein-coupled receptors: orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). Orexins, along with orexin receptors, are expressed in retinal neurons, and they have been shown to differentially modulate excitatory AMPA receptors of amacrine and ganglion cells in the inner retina. In this work we report that orexin-B modulates the activity of rod bipolar cells (RBCs) located in the outer retina of rat. Intravitreal injection of orexin-B increased the amplitude of the scotopic electroretinographic b-wave, a reflection of RBC activity, recorded in vivo. Patch clamp recordings in rat retinal slices showed that orexin-B did not change glutamatergic excitatory component of the RBC response driven by photoreceptors. Effects of orexin-B on GABA receptor-mediated synaptic transmission of RBCs were then examined. In retinal slice preparations orexin-B suppressed GABA receptor-mediated inhibitory postsynaptic currents of RBCs in the inner plexiform layer. Furthermore, using whole-cell recordings in isolated RBCs it was shown that orexin-B suppressed GABAC receptor-, but not GABAA receptor-, mediated currents of the RBCs, an effect that was blocked by OX1R and OX2R antagonists. The orexin-B-induced inhibition of GABAC currents was likely mediated by a Gi/o/PC-PLC/Ca2+-independent PKC signaling pathway, as such inhibition was absent when each step of the above-pathway was blocked with GDP-β-S/pertussis toxin (for Gi/o), D609 (for PLC), bisindolylmaleimide IV (for PKC)/rottlerin (for PKCδ), respectively. The orexin-B-induced potentiation of RBC activity may improve visual acuity and contrast sensitivity of the animal during the dark period (wake phase).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 133, 1 May 2018, Pages 38-50
Journal: Neuropharmacology - Volume 133, 1 May 2018, Pages 38-50
نویسندگان
Gong Zhang, Xiao-Hua Wu, Guo-Zhong Xu, Shi-Jun Weng, Xiong-Li Yang, Yong-Mei Zhong,