کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8526394 | 1557945 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Notch signaling pathway dampens tumor-infiltrating CD8+ T cells activity in patients with colorectal carcinoma
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DAPTT-cell immunoglobulin and mucin-domain containing-3CTLA-4UICCgranulocyte-macrophage colony stimulating factorGM-CSFPBMCsCCK-8IFN-γNCSSPSSRT-PCRPD-1FBSCD8+ T cells - CD8 + سلول های TUnion Internationale Contre le Cancer - اتحادیه بین المللی سرطانinterferon-γ - اینترفرون-γinterleukin - اینترلوکینTumor-infiltrating lymphocytes - تومور لنفوسیتها نفوذ می کندtumor necrosis factor-α - تومور نکروز عامل αTim-3 - تیم 3TILS - جریان بداخلStatistical Product and Service Solutions - راه حل های محصولات و خدمات آماریcolorectal carcinoma - سرطان روده بزرگ و مقعدfetal bovine serum - سرم جنین گاوperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیcell counting kit-8 - شمارش سلول کیت 8Vascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)TNF-α - فاکتور نکروز توموری آلفاlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Programmed death-1 - مرگ برنامه ریزی شده 1Notch signaling - نشانه گیریreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیnormal controls - کنترل های نرمال
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
CD8+ T cells play critical role in controlling the metastasis and prognosis of cancer. Controversy remains as to the contribution of Notch signaling pathway in modulation of CD8+ T cells activity and development of tumorigenesis. Thus, the aim of the current study was to investigate the immunoregulatory role of Notch signaling pathway to peripheral and tumor-infiltrating CD8+ T cells in patients with colorectal carcinoma. A total of 46 patients with colorectal carcinoma and 20 health individuals were enrolled, and CD8+ T cells were purified from both peripheral bloods and carcinoma specimens. Cytolytic and noncytolytic functions of CD8+ T cells in response to Notch signaling inhibition were evaluated by measurements of lactate dehydrogenase release and proinflammatory cytokines production in both direct and indirect contact co-culture system to target HT29 cells. Cellular proliferation and inhibitory receptors expression in CD8+ T cells were also assessed by CCK-8 method and flow cytometry. There was no remarkable difference in percentage of CD8+ T cells between healthy individuals and patients with colorectal carcinoma. Notch1/2 and Hes1/5 mRNAs were elevated expressed in tumor-infiltrating CD8+ T cells in patients with colorectal carcinoma, however, did not correlated with tumor differentiation or stages. CD8+ T cells from healthy individuals presented stronger cytotoxicity, which was not affected by Notch signaling inhibitor. Inhibition of Notch signaling pathway not only promoted cytotoxicity of tumor-infiltrating CD8+ T cells, but also enhanced proinflammatory cytokines (including IFN-γ, TNF-α, IL-1β, IL-6, and IL-8) production by CD8+ T cells from patients with colorectal carcinoma. This process was accompanied by decreased expression of PD-1 in CD8+ T cells without influencing cellular proliferation. Our results indicated a potential immunosuppressive property of Notch signaling pathway, which dampened both cytolytic and noncytolytic functions of CD8+ T cells probably via induction of PD-1 in patients with colorectal carcinoma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 97, January 2018, Pages 535-542
Journal: Biomedicine & Pharmacotherapy - Volume 97, January 2018, Pages 535-542
نویسندگان
Weifeng Yu, Yanjun Wang, Peng Guo,