کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8768990 | 1597917 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect
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کلمات کلیدی
S1PIL-1βPBSS1PRMVSMicrovesiclesMMP2FBSRT-PCRFITCMSCsGFPGAPDH5-ethynyl-2′-deoxyuridine - 5-ethynyl-2'-deoxyuridineEdU - EDUH&E - H & ELC/MS - LC / MSSphingosine-1-phosphate - اسپینگسین-1-فسفاتperiodic acid-Schiff - اسید فسفریک SchiffTem - این استinterleukin 1 beta - اینترلوکین 1 بتاfetal bovine serum - سرم جنین گاوMesenchymal stem cells - سلول های بنیادی مزانشیمیLiquid chromatography-mass spectrometry - طیف سنجی جرم کروماتوگرافی مایعPhosphate buffered saline - فسفات بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتmatrix metalloproteinase 2 - ماتریکس متالوپروتئیناز 2Transmission electron microscopy - میکروسکوپ الکترونی عبوریPAS - نهHematoxylin and Eosin - هماتوکسیلین و ائوزینReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایgreen fluorescent protein - پروتئین فلورسنت سبزPropidium iodide - پروتئین یدیدhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازS1P receptor - گیرنده S1P
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect](/preview/png/8768990.png)
چکیده انگلیسی
Microvesicles (MVs) are emerging as a new mechanism of intercellular communication by transferring cellular components to target cells, yet their function in disease is just being explored. However, the therapeutic effects of MVs in cartilage injury and degeneration remain unknown. We found MVs contained high levels of sphingosine-1-phosphate (S1P) compared with the original bone marrow mesenchymal stem cells (MSCs). The enrichment of S1P in MVs was mediated by sphingosine kinase 1 (SphK1), but not by sphingosine kinase 2 (SphK2). Co-culture of human chondrocytes with MVs resulted in increased proliferation of chondrocytes in vitro, which was mediated by activation of S1P receptor 1 (S1PR1) expressed on chondrocytes. Meanwhile, MVs inhibited interleukin 1 beta-induced human chondrocytes apoptosis in a dose dependent manner. Furthermore, uptake of MVs by primary cultures of human chondrocytes was mediated by CD44 expressed by MVs. Anti-CD44 antibody significantly reduced the uptake of fluorescent protein-labeled MVs by chondrocytes. Further, blocking S1P by its neutralizing antibody significantly inhibited the therapeutic effects of MVs in vivo. Taken together, MVs showed therapeutic potential for treatment of clinical cartilage injury. This therapeutic potential is due to CD44-mediated uptake of MVs by chondrocytes and the S1P/S1PR1 axis-mediated proliferative effects of MVs on chondrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 193, March 2018, Pages 42-53
Journal: Translational Research - Volume 193, March 2018, Pages 42-53
نویسندگان
Chuan Xiang, Kun Yang, Zhiyong Liang, Yulong Wan, Yanwei Cheng, Dong Ma, Heng Zhang, Weiyu Hou, Panfeng Fu,