کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840627 | 1614692 | 2018 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Monoclonal Antibody DL11C8 Identifies ADAM23 as a Component of Lipid Raft Microdomains
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کلمات کلیدی
TNFADAM23ConAPrPcFCSWGAMonoclonal antibody - آنتی بادی مونوکلونالDisintegrins - انحلالIntegrin - اینتگرینELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاCNS - دستگاه عصبی مرکزیfetal calf serum - سرم گوساله جنینcentral nervous system - سیستم عصبی مرکزیtumor necrosis factor - فاکتور نکروز تومورPrion protein - پروتئین پریونcellular prion protein - پروتون پریون سلولیLipid rafts - گستره چربی، کلک چربی یا لیپید رفتWheat germ agglutinin - گیاه گندم آگلوتیینین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A disintegrin and metalloprotease protein 23 (ADAM23) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors. Here, we describe a novel monoclonal antibody (DL11C8) that specifically recognizes the cysteine-rich domain of both pre-protein (100â¯kDa) and mature (70â¯kDa) forms of ADAM23 from different species, including human, rodents and avian orthologs. Using this antibody, we detected both forms of ADAM23 on the cell surface of three neuronal cell lineages (Neuro-2a, SH-SY5Y and CHLA-20), with a higher relative content of ADAM23100â¯kDa. Furthermore, we demonstrate for the first time that a catalytically inactive member of the ADAM family is present in the membrane signaling platforms, namely lipid rafts. Indeed, the mature ADAM2370â¯kDa partitions between raft and non-raft membrane domains, while the pro-protein ADAM23100â¯kDa is mainly expressed in non-raft domains. These membranous distributions were observed in both different brain regions homogenates and primary cultured neurons lysates from mouse cortex and cerebellum. Taken together, these findings point out ADAM23 as a lipid raft molecular component.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 384, 1 August 2018, Pages 165-177
Journal: Neuroscience - Volume 384, 1 August 2018, Pages 165-177
نویسندگان
Zaine L.M. Borgonovo, Caroline F. Ribeiro, Michele D.M. Costa, Ingrid L.M. Souza, Gustavo R. Rossi, Monica V. Alcantara, Max Ingberman, Luciano G. Braga, Adriana F. Mercadante, Lia S. Nakao, Silvio M. Zanata,