کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8850755 | 1618763 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitochondrial apoptotic pathway mediated the Zn-induced lipolysis in yellow catfish Peteobagrus fulvidraco
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کلمات کلیدی
GAPDHUBCEMPTAPAf-1PPARαribosomal protein L7RPL7HprtTATA-box-binding proteinCPT INACHSLCYCSTBPCASP9MMPb2mN-acetylcysteine - N-استیل سیستئینROS - ROSz-VAD-fmk - Z-VAD-FMKubiquitin-conjugating enzyme - آنزیم کنگوتیک ubiquitinAtgl - اتگلelfa - الفاmitochondrial permeability transition - انتقال نفوذپذیری میتوکندریCSA - ایالات مؤتلفهٔ آمریکاBeta-2-microglobulin - بتا-2-میکروگلوبولینtriglyceride - تریگلیسریدTuba - توباApoptosis - خزان یاختهایcytochrome c - سیتوکروم سیcyclosporin A - سیکلوسپورین Atranslation elongation factor - ضریب اصطکاک ترجمهZinc - فلز رویhormone-sensitive lipase - لیپاز حساس به هورمونLipid metabolism - متابولیسم لیپیدMitochondrial apoptotic pathway - مسیر آپوپتوتیک میتوکندریایhypoxanthine-guanine phosphoribosyltransferase - هیپوکسانتین-گوانین فسفریبوسیل ترانسفرازMitochondrial membrane potential - پتانسیل غشای میتوکندریCarnitine palmitoyltransferase 1 - کارنتین پالمیتویل ترانسفراز 1Caspase 9 - کاسپاز 9glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the study, effects of waterborne zinc (Zn) exposure on apoptosis were investigated, and the potential mechanism of apoptosis participating in the Zn-induced variations of lipid metabolism was explored in a low vertebrate, yellow catfish Pelteobagrus fulvidraco. We found that Zn induced occurrence of apoptosis of livers and hepatocytes in yellow catfish. Waterborne Zn also increased hepatic transcriptional levels of p53, cytochrome c (Cycs), caspase 3a (Casp3a) and caspase 3b (Casp3b) of yellow catfish. Zn increased caspase 3 activity and reduced the mitochondrial permeability transition (MTP) in yellow catfish hepatocytes. Z-VAD-fmk (caspase inhibitor) and CsA pretreatment (MTP inhibitor) attenuated the Zn-induced apoptosis and reduction in MTP. Z-VAD-fmk pretreatments attenuated the Zn-induced increase in transcriptional levels of p53, Cycs and Casp3b although the differences were not statistically significant between the Zn group and Zn + Z-VAD-fmk group. In contrast, Zn and N-acetylcysteine (NAC) did not significantly influence the reactive oxygen species (ROS) production. Zn significantly reduced triglyceride (TG) content, increased the activities of carnitine palmitoyltransferase 1 (CPT I), hormone-sensitive lipase (HSL) and adipose TAG lipase (ATGL), and the transcriptional levels of p53, Cycs and caspase 3b of the hepatocytes; these Zn-induced effects on TG contents, activities of CPT I, HSL and ATGL, and mRNA levels of p53, Cycs and caspase 3b could partly be reversed by Z-VAD-fmk, suggesting that Zn induced the mitochondrial-mediated apoptosis and reduced lipid accumulation. Taken together, our study demonstrated the importance of mitochondria-mediated apoptosis in Zn-induced lipolysis, which suggested a new mechanism for elucidating metal element influencing lipid metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 208, October 2018, Pages 907-915
Journal: Chemosphere - Volume 208, October 2018, Pages 907-915
نویسندگان
Dan-Dan Li, Zhi Luo, Shi-Cheng Ling, Kun Wu, Guang-Hui Chen, Jie Cheng,