کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8881389 | 1624882 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of the natural dehydration mechanism during middle and late stages of wheat seeds development by some physiological traits and iTRAQ-based proteomic
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کلمات کلیدی
DAADifferentially abundant proteinsMDARPLCITRAQDAPsLEAFDRCATPODKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوDevelopment - رشدdays after anthesis - روز بعد از آنتی ژنLate embryogenesis abundant - زودرس زودرس فراوان استSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازmalondialdehyde - مالون دی آلدهیدfalse discovery rate - میزان کشف کاذبPeroxidase - پراکسیدازCatalase - کاتالازReversed phase liquid chromatography - کروماتوگرافی مایع فاز برگشتیWheat - گندم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Jimai 22 wheat seeds collected at eight developmental stages [20-38 days after anthesis (daa)] were used to characterize the natural dehydration of wheat seeds. An iTRAQ-based quantitative proteomics technique was applied to identify differentially abundant proteins (DAPs) and evaluate several physiological traits at 20, 26, 32 and 38 daa. Superoxide dismutase and peroxidase activities as well as soluble sugar and soluble protein contents increased during dehydration, while the malondialdehyde content decreased. These results suggested that the antioxidant capacity of seeds increased during the dehydration process. A total of 128 proteins exhibited at least a 1.5-fold difference in abundance relative to the control levels in naturally dehydrating seeds. Forty of the DAPs were related to defense/stress responses. We observed an increase in the abundance of five late embryogenesis abundant proteins, five serpins, a heat shock cognate 70-kDa protein, and the 17.7-kDa Class I heat shock protein, with peak levels detected at 38 daa. Additionally, starch and sucrose metabolic activities were also associated with the natural dehydration of wheat seeds, with trehalose-6-phosphate synthase 7 increasing in abundance during the dehydration process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cereal Science - Volume 80, March 2018, Pages 102-110
Journal: Journal of Cereal Science - Volume 80, March 2018, Pages 102-110
نویسندگان
Lulu Chen, Zhenlin Wang, Meiling Li, Xueli Ma, Enyun Tian, Aiqing Sun, Yanping Yin,