کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8887989 | 1628375 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prophage induction reduces Shiga toxin producing Escherichia coli (STEC) and Salmonella enterica on tomatoes and spinach: A model study
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fresh produce is increasingly implicated in foodborne outbreaks and most fresh produce is consumed raw, emphasizing the need to develop non-thermal methods to control foodborne pathogens. This study investigates bacterial cell lysis through induction of prophages as a novel approach to control foodborne bacterial pathogens on fresh produce. Shiga toxin producing Escherichia coli (STEC) and Salmonella enterica isolates were exposed to different prophage inducers (i.e. mitomycin C or streptonigrin) and growth of the cells was monitored by measuring the optical density (OD600) during incubation at 37â¯Â°C. Beginning at three hours after addition of the inducer, all concentrations (0.5, 1, 2â¯Î¼g/mL) of mitomycin C, or 2â¯Î¼g/mL streptonigrin significantly reduced the OD600 in broth cultures, in a concentration dependent manner, relative to cultures where no inducer was added. PCR confirmed bacterial release of induced bacteriophages and demonstrated that a single compound could successfully induce multiple types of prophages. The ability of mitomycin C to induce prophages in STEC O157:H7 and in S. enterica (serovars Typhimurium and Newport) on fresh produce was evaluated by inoculating red greenhouse tomatoes or spinach leaves with 5â¯Ãâ¯107 and 5â¯Ãâ¯108 colony forming units, respectively. After allowing time for the inoculum to dry on the fresh produce samples, 6â¯Î¼g/mL mitomycin C was sprayed onto each sample, while control samples were sprayed with water. Following overnight incubation at 4â¯Â°C, the bacterial cells were recovered and plate counts were performed. A 3 log reduction in STEC O157:H7 cells was observed on tomatoes sprayed with mitomycin C compared to those sprayed with water, while a 1 log reduction was obtained on spinach. Similarly, spraying mitomycin C on tomatoes and spinach inoculated with S. enterica isolates resulted in a 1-1.5 log and 2 log reduction, respectively. These findings serve as a proof of concept that prophage induction can effectively control bacterial foodborne pathogens on fresh produce.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 89, July 2018, Pages 250-259
Journal: Food Control - Volume 89, July 2018, Pages 250-259
نویسندگان
Brigitte Cadieux, Anna Colavecchio, Julie Jeukens, Luca Freschi, Jean-Guillaume Emond-Rheault, Irena Kukavica-Ibrulj, Roger C. Levesque, Sadjia Bekal, Jeffrey C. Chandler, Shannon M. Coleman, Bledar Bisha, Lawrence D. Goodridge,