کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8978164 | 1106579 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Catfish hepcidin gene is expressed in a wide range of tissues and exhibits tissue-specific upregulation after bacterial infection
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کلمات کلیدی
RT-PCRdeoxycytosine triphosphateUTRMS 222Enteric septicemia of catfishEdwardsielladCTPdATPBHIAMPIL-6CFUcDNA - cDNAComplementary DNA - DNA تکمیلیBAC - LACEST - استUltraviolet - اشعه فرابنفشInnate immunity - ایمنی ذاتیinterleukin-6 - اینترلوکین ۶Gene expression - بیان ژنDisease - بیماریtricaine methanesulfonate - تری کینین متانسولفوناتbrain heart infusion - تزریق قلب مغزیExpressed sequence tags - تگ های توالی بیان شدهESC - خروجdeoxyadenosine triphosphate - دایکا سوزیدنوسین تری فسفاتInfection - عفونتFish - ماهیuntranslated region - منطقه غیر ترجمهHepcidin - هپسیدین colony forming units - واحدهای تشکیل دهنده کلنیReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیAntimicrobial peptide - پپتیدهای ضدمیکروبیAntimicrobial peptides - پپتیدهای پادمیکرب یا آنتیمایکروبیال پپتایدSNP - چندریختی تک-نوکلئوتیدbacterial artificial chromosome - کروموزوم مصنوعی باکتریاییCatfish - گربه ماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. The cysteine-rich AMPs such as defensin and hepcidin have been extensively studied from various organisms, but their role in disease defense in catfish is unknown. As a first step, we sequenced a hepcidin cDNA from both channel catfish and blue catfish, and characterized the channel catfish hepcidin gene. The channel catfish hepcidin gene consists of two introns and three exons that encode a peptide of 96 amino acids. The amino acid sequences and gene organization were conserved between catfish and other organisms. In contrast to its almost exclusive expression in the liver in humans, the channel catfish hepcidin gene was expressed in a wide range of tissues except brain. Its expression was detected early during embryonic and larval development, and induced after bacterial infection with Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish (ESC) in a tissue-specific manner. The upregulation was observed in the spleen and head kidney, but not in the liver. The expression of hepcidin was upregulated 1-3 days after challenge, but returned to normal levels at 7 days after challenge. The expression profile of the catfish hepcidin gene during the course of bacterial infection mirrors those of inflammatory proteins such as chemokines, suggesting an important role for hepcidin during inflammatory responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 29, Issue 11, 2005, Pages 939-950
Journal: Developmental & Comparative Immunology - Volume 29, Issue 11, 2005, Pages 939-950
نویسندگان
Baolong Bao, Eric Peatman, Ping Li, Chongbo He, Zhanjiang Liu,