کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8989619 | 1555475 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys (M. fascicularis)
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کلمات کلیدی
16S ribosomal RNA - RNA ریبوزوم 16SDNA fingerprinting - اثر انگشت دیجیتالGenotyping - تعیین ساختار ژنتیکیDNA sequencing - توالی یابی DNA، تعیین توالی دیانایGastric erosions - فرسایش معدهAnimal model - مدل حیوانیStomach - معده یا شکمcynomolgus monkey - میمون cynomolgusHelicobacter pylori - هلیکوباکتر پیلوریNonhuman primate - پریمات غیر انسانیGastritis - گاستریت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys (M. fascicularis) Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys (M. fascicularis)](/preview/png/8989619.png)
چکیده انگلیسی
We recently reported the occurrence of natural infection with H. pylori in a group of cynomolgus monkeys with chronic active gastritis and gastric erosions. The goal of the present study was to characterize and to compare strains isolated from animals originating from two different geographical areas. Gross and microscopic pathology determined at the time of necropsy was similar in all animals. H. pylori were isolated from specimens harvested in five monkeys (four from Vietnam and one from the Philippines) with gastritis. Isolates from monkeys bred in Vietnam had a similar DNA fingerprint pattern, which was distinct from that of isolates from a monkey bred in the Philippines. All strains were of the s1a vacA subtype, but all the 'Vietnamese' strains were cagA+ and all but one were iceA1 whereas the 'Philippino' strains were cagAâ and iceA2. The sequences of the 16S rRNA of the Vietnamese and Philippino strains shared 98% homology and both clustered with H. pylori sequences present in the NCBI database. In conclusion, cynomolgus monkeys can be naturally colonized by H. pylori, and the strains isolated from these animals appear to vary according to the geographical origin, thus indicating probable infection prior to importation. Since some of the cynomolgus monkeys developed antral erosions during natural infection, we propose that this animal model may be used to investigate the role of H. pylori in ulcerogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 108, Issues 1â2, 15 June 2005, Pages 133-139
Journal: Veterinary Microbiology - Volume 108, Issues 1â2, 15 June 2005, Pages 133-139
نویسندگان
Sonia Q. Doi, Tara Kimbason, James Reindel, Andre Dubois,