کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002032 | 1118569 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The role of thioredoxin reductase activity in selenium-induced cytotoxicity
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کلمات کلیدی
GPXDTNBTrxHEK-293TrxR1Cell motility - تحرک سلولیthioredoxin reductase - تریودوکسین ردوکتازThioredoxin reductase 1 - تریوردوکسین ردوکتاز 1Oxidative stress - تنش اکسیداتیوthioredoxin - تیرودوکسینSelenium - سلنیومCytotoxicity - سمیت سلولیglutathione reductase - گلوتاتیون ردوکتازglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The role of thioredoxin reductase activity in selenium-induced cytotoxicity The role of thioredoxin reductase activity in selenium-induced cytotoxicity](/preview/png/9002032.png)
چکیده انگلیسی
The selenoprotein thioredoxin reductase is a key enzyme in selenium metabolism, reducing selenium compounds and thereby providing selenide to synthesis of all selenoproteins. We evaluated the importance of active TrxR1 in selenium-induced cytotoxicity using transfected TrxR1 over-expressing stable Human Embryo Kidney (HEK-293) cells and modulation of activity by pretreatment with low concentration of selenite. Treatment with sodium selenite induced cytotoxity in a dose-dependent manner in both TrxR1 over-expressing and control cells. However, TrxR1 over-expressing cells, which were preincubated for 72 h with 0.1 μM selenite, were significantly more resistant to selenite cytotoxicity than control cells. To demonstrate the early effects of selenite on behaviour of HEK-293 cells, we also investigated the influence of this compound on cell motility. We observed inhibition of cell motility by 50 μM selenite immediately after administration. Moreover, TrxR1 over-expressing cells preincubated with a low concentration of selenite were more resistant to the inhibitory effect of 50 μM selenite than those not preincubated. It was also observed that the TrxR over-expressing cells showed higher TrxR1 activity than control cells and the preincubation of over-expressing cells with 0.1 μM selenite induced further significant increase in the activity of TrxR1. On the other hand, we demonstrated that TrxR1 over-expressing cells showed decreased glutathione peroxidase activity compared to control cells. These data strongly suggest that TrxR1 may be a crucial enzyme responsible for cell resistance against selenium cytotoxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 12, 15 June 2005, Pages 1765-1772
Journal: Biochemical Pharmacology - Volume 69, Issue 12, 15 June 2005, Pages 1765-1772
نویسندگان
Zbigniew Madeja, Jolanta Sroka, Christina Nyström, Linda Björkhem-Bergman, Tomas Nordman, Anastasios Damdimopoulos, Ivan Nalvarte, Lennart C. Eriksson, Giannis Spyrou, Jerker M. Olsson, Mikael Björnstedt,