کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038658 | 1133855 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite
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کلمات کلیدی
FenvalerateTBARS, thiobarbituric acid reactive speciesTEP, 1,1,3,3-tetraethoxypropaneLPO, lipid peroxidation - LPO، پراکسیداسیون لیپیدMDA, malondialdehyde - MDA، مالون دی آلدئیدOS, oxidative stress - OS، استرس اکسیداتیوROS, Reactive Oxygen Species - ROS، انواع اکسیژن واکنشیAntioxidant enzymes - آنزیم های آنتی اکسیدانErythrocytes - اریتروسیت هاOxidative stress - تنش اکسیداتیوDMSO, dimethyl sulfoxide - دیمتیل سولفواکسیدSOD, superoxide dismutase - سوپراکسید دیسموتازScanning electron microscopy - میکروسکوپ الکترونی روبشیGR, glutathione reductase - گلوتاتیون ردوکتازGPX, glutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Erythrocytes are a convenient model to understand the membrane oxidative damage induced by various xenobiotic-prooxidants. In this investigation, we have examined the potency of Fenvalerate (FEN) and its metabolite, p-chlorophenyl isovaleric acid (p-CPIA) to induce oxidative stress response in rat erythrocytes in vitro in terms of lipid peroxidation and effects on selected antioxidant enzymes. Susceptibility of erythrocytes to FEN exposure was further investigated in terms of morphological alterations by scanning electron microscopy and protein damage by gel electrophoresis of erythrocyte ghosts. Following in vitro exposure, FEN caused a significant induction of oxidative damage in erythrocytes at concentrations beyond 0.1Â mM as evidenced by increased thiobarbituric acid reactive substances (TBARS) levels. The response was both concentration and time dependent. At higher concentrations, significant decreases in the activities of vital antioxidant enzymes viz., catalase, superoxide dismutase, glutathione transferase and glutathione reductase were also discernible clearly suggesting the potency of both, parent compound and its metabolite to induce oxidative stress in erythrocytes. Scanning electron micrographs of erythrocytes following FEN exposure at higher concentrations revealed various degrees of distortion in shape and ruptured membranes. Furthermore, gel electrophoresis studies revealed consistent and significant aggregation of only band 3 protein in erythrocyte membranes exposed to either FEN or p-CPIA at higher concentrations. These in vitro findings show that FEN and its metabolite have the propensity to cause significant oxidative damage in rat erythrocytes, which is associated with marked damage to membrane proteins. These data suggest that both structural and functional perturbations may ensue in erythrocytes following exposure to FEN at higher concentrations under in vivo situations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 4, June 2005, Pages 449-456
Journal: Toxicology in Vitro - Volume 19, Issue 4, June 2005, Pages 449-456
نویسندگان
K. Prasanthi, Muralidhara Muralidhara, P.S. Rajini,