کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9132059 | 1160982 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multiple transcripts of sodium channel SCN8A (NaV1.6) with alternative 5â²- and 3â²-untranslated regions and initial characterization of the SCN8A promoter
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کلمات کلیدی
CPMUTRRE1-silencing transcription factorSCN8APolyadenylationCPEBAC - LACrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدconserved sequence - توالی محافظت شدهembryonic day - روز جنینیpostnatal day - روز پس از زایمانTranscription initiation site - سایت راه اندازی رونویسیcounts per minute - شمار در هر دقیقهcytoplasmic polyadenylation element - عنصر polyadenylation سیتوپلاسمیکSp1 transcription factor - فاکتور رونویسی Sp1Race - مسابقه3′-untranslated regions - مناطق 3'-غیر ترجمه شدهuntranslated region - منطقه غیر ترجمهbacterial artificial chromosome - کروموزوم مصنوعی باکتریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Multiple transcripts of sodium channel SCN8A (NaV1.6) with alternative 5â²- and 3â²-untranslated regions and initial characterization of the SCN8A promoter Multiple transcripts of sodium channel SCN8A (NaV1.6) with alternative 5â²- and 3â²-untranslated regions and initial characterization of the SCN8A promoter](/preview/png/9132059.png)
چکیده انگلیسی
To identify the transcriptional start sites of the neuronal channel SCN8A, we carried out 5â²-RACE (rapid amplification of cDNA ends) with RNA from human and mouse brain. We recovered four mutually exclusive 5â²-untranslated exons (exon 1a to exon 1d) that map to a 1.8-kb region of genomic DNA located â¼70 kb upstream of the first coding exon. The same 5â²-untranslated exons are expressed in central, peripheral and sympathetic nervous system and in embryonic and adult brain. A 4.8-kb genomic fragment containing these 5â² exons demonstrated promoter activity in transfected MN-1 cells. In transgenic mice, transcription of the 4.8-kb promoter was restricted to brain and spinal cord. The 4.8-kb promoter contains eight consensus Sp1-binding sites and two Inr sites. A potential NRSE/RE-1 site is located nearby. Two active polyadenylation sites identified by 3â²-RACE are conserved in human, mouse, and chicken SCN8A. Sequence comparison of human and mouse SCN8A identified 12 conserved noncoding elements whose effect on transcription was tested in transfected cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 85, Issue 2, February 2005, Pages 245-257
Journal: Genomics - Volume 85, Issue 2, February 2005, Pages 245-257
نویسندگان
Valerie L. Drews, Andrew P. Lieberman, Miriam H. Meisler,