کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9191851 | 1186601 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative analysis of the generation of different striatal neuronal subtypes in the adult brain following excitotoxic injury
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کلمات کلیدی
PBSSVZKPBSMCAONeuNNPYPFADcxDARPP-325-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز γ-aminobutyric acid - اسید γ-آمینوبوتیریکQuinolinic acid - اسید کینولینیکmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیBrdU - بروموداکسی اوریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancedoublecortin - دوچرخهBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریsubventricular zone - منطقه فرعیRat striatum - موش صحراییNeurogenesis - نوروژنزparaformaldehyde - پارافرمالدهیدParv - پاروParvalbumin - پاروالبومینpotassium phosphate-buffered saline - پتاسیم فسفات بافر شورChAT - چتcholine acetyltransferase - کولین استیل ترانسفرازGABA - گاباNeuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recent findings in adult rodents have provided evidence for the formation of new striatal neurons from subventricular zone (SVZ) precursors following stroke. Little is known about which factors determine the magnitude of striatal neurogenesis in the damaged brain. Here we studied striatal neurogenesis following an excitotoxic lesion to the adult rat striatum induced by intrastriatal quinolinic acid (QA) infusion. New cells were labeled with the thymidine-analogue 5-bromo-2â²-deoxyuridine (BrdU) and their identity was determined immunocytochemically with various phenotypic markers. The unilateral lesion gave rise to increased cell proliferation mainly in the ipsilateral SVZ. At 2 weeks following the insult, there was a pronounced increase of the number of new neurons co-expressing BrdU and a marker of migrating neuroblasts, doublecortin, in the ipsilateral striatum, particularly its non-damaged medial parts. About 80% of the new neurons survived up to 6 weeks, when they expressed the mature neuronal marker NeuN and were preferentially located in the outer parts of the damaged area. Lesion-generated neurons expressed phenotypic markers of striatal medium spiny neurons (DARPP-32) and interneurons (parvalbumin or neuropeptide Y). The magnitude of neurogenesis correlated to the size of the striatal damage. Our data show for the first time that an excitotoxic lesion to the striatum can trigger the formation of new striatal neurons with phenotypes of both projection neurons and interneurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 195, Issue 1, September 2005, Pages 71-80
Journal: Experimental Neurology - Volume 195, Issue 1, September 2005, Pages 71-80
نویسندگان
Tove Collin, Andreas Arvidsson, Zaal Kokaia, Olle Lindvall,