کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9230287 | 1203624 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Retinoic Acid and its 4-Oxo Metabolites are Functionally Active in Human Skin Cells In Vitro
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Retinoic Acid and its 4-Oxo Metabolites are Functionally Active in Human Skin Cells In Vitro Retinoic Acid and its 4-Oxo Metabolites are Functionally Active in Human Skin Cells In Vitro](/preview/png/9230287.png)
چکیده انگلیسی
Retinoic acid exerts a variety of effects on gene transcription that regulate growth, differentiation, and inflammation in normal and neoplastic skin cells. Because there is a lack of information regarding the influence of metabolic transformation of retinoids on their pharmacologic effects in skin, we have analyzed the functional activity of all-trans-, 9-cis-, and 13-cis-retinoic acid and their 4-oxo-metabolites in normal human epidermal keratinocytes (NHEKs) and dermal fibroblasts using gene and protein expression profiling techniques, including cDNA microarrays, two-dimensional gel electrophoresis, and MALDI-MS. It was previously thought that the 4-oxo-metabolites of RA are inert catabolic end-products but our results indicate instead that they display strong and isomer-specific transcriptional regulatory activity in both NHEKs and dermal fibroblasts. Microarray and proteomic analyses identified a number of novel genes/gene products that are influenced by RA treatment of NHEKs or fibroblasts, including genes for enzymes catalyzing biotransformation of retinoids, corticosteroids, and antioxidants and structural and transport proteins known to be essential for homeostasis. Our results expand current knowledge regarding retinoic acid action within skin cells and the target tissue/cell regulatory systems that are important for modulating the physiological and pharmacological effects of this important class of dermatological drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 125, Issue 1, July 2005, Pages 143-153
Journal: Journal of Investigative Dermatology - Volume 125, Issue 1, July 2005, Pages 143-153
نویسندگان
Jens M. Baron, Ruth Heise, William S. Blaner, Mark Neis, Sylvia Joussen, Alexandra Dreuw, Yvonne Marquardt, Jean-Hilaire Saurat, Hans F. Merk, David R. Bickers, Frank K. Jugert,