کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9230592 | 1203642 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteolytic Processing of the Laminin α3 G Domain Mediates Assembly of Hemidesmosomes but Has No Role on Keratinocyte Migration
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
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![عکس صفحه اول مقاله: Proteolytic Processing of the Laminin α3 G Domain Mediates Assembly of Hemidesmosomes but Has No Role on Keratinocyte Migration Proteolytic Processing of the Laminin α3 G Domain Mediates Assembly of Hemidesmosomes but Has No Role on Keratinocyte Migration](/preview/png/9230592.png)
چکیده انگلیسی
Laminin-5 (Lm5), the major adhesion ligand of basal epithelial cells, undergoes complex extracellular proteolytic processing that influences cell adhesion and migration. In tumor cell lines, the proteolytic truncation of the C-terminal G domain of the Lm α3 chain induces assembly of hemidesmosomes and downregulates cell migration. To define the biological functions of the α3 G domain processing in physiological conditions, we have expressed a series of mutant α3 complementary DNA in human primary α3-null keratinocytes immortalized by human papillomavirus E6E7 (HKα3 cells). Using monolayer and organotypic cell cultures we show that: (1) the hinge region between subdomains G3 and G4 carries the proteolytic cleavage sites; (2) nucleation of the hemidesmosomal proteins is independent of the proteolytic maturation of the α3 G domain, whereas formation of mature hemidesmosomes relies on proteolytic cleavage of α3; and (3) the proteolytic processing plays no role in cell migration, which suggests that nucleation of hemidesmosomal structures in culture does not reflect the migratory potential of the epithelial cells. Our results also demonstrate that HKα3 cells are a unique model system, which will be useful to dissect the functions and molecular interactions of Lm5.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 125, Issue 5, November 2005, Pages 883-888
Journal: Journal of Investigative Dermatology - Volume 125, Issue 5, November 2005, Pages 883-888
نویسندگان
Christian Baudoin, Laurence Fantin, Guerrino Meneguzzi,