کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9244066 | 1209899 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Melastatin-Type Transient Receptor Potential Channel 7 Is Required for Intestinal Pacemaking Activity
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کلمات کلیدی
PIP2TRPCTrpICCGTLinterstitial cell of CajalHEKNSCCTRPMRT-PCR2-aminoethoxydiphenyl borate2-APBTRPVRNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNAGastrointestinal - دستگاه گوارشphosphatidylinositol 4,5-bisphosphate - فسفاتیدیلینوزیتول 4،5-بیسفسفاتpolymerase chain reaction - واکنش زنجیره ای پلیمرازreverse-transcription polymerase chain reaction - واکنش زنجیره پلیمراز معکوس رونویسیPCR - واکنش زنجیرهٔ پلیمرازtransient receptor potential - پتانسیل گیرنده گذراNonselective cation channel - کانال غیر کیهانیhuman embryonic kidney - کلیه جنین انسان
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: Interstitial cells of Cajal are pacemakers in the gastrointestinal tract, regulating rhythmicity by activating nonselective cation channels. In Caenorhabditis elegans, the melastatin-type transient receptor potential (TRPM) channel, especially TRPM7, was suggested as being involved in defecation rhythm. The aim here was to show that the nonselective cation channel in interstitial cells of Cajal in mouse small intestine has properties essentially identical to those of murine TRPM7, heterologously expressed in human embryonic kidney cells. Methods: The patch-clamp technique for whole-cell recording was used in cultured or single interstitial cells of Cajal. TRPM7-specific small interfering RNAs were used for specific inhibition of TRPM7. Results: Electrophysiological and pharmacological properties of the nonselective cation channel in interstitial cells of Cajal were the same as those of TRPM7. Reverse-transcription polymerase chain reaction, Western blotting, and immunohistochemistry all showed abundant and localized expression of TRPM7 messenger RNA and protein in mouse small intestine. Treatment of primary cultured interstitial cells of Cajal with TRPM7-specific small interfering RNA resulted in inhibition of pacemaking activity. Conclusions: TRPM7 is required for intestinal pacemaking. The protein is a likely potential target for pharmacological treatment of motor disorders of the gut.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 129, Issue 5, November 2005, Pages 1504-1517
Journal: Gastroenterology - Volume 129, Issue 5, November 2005, Pages 1504-1517
نویسندگان
Byung Joo Kim, Hyun-Ho Lim, Dong Ki Yang, Jae Yeoul Jun, In Youb Chang, Chul-Seung Park, Insuk So, Peter R. Stanfield, Ki Whan Kim,