کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9266231 | 1217274 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
IgM production of lymphocytes from C57BL/6N mice was stimulated by estrogen treated splenic adherent cells
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کلمات کلیدی
E2-BSAPBSDPNLPSPPTFBSFITC17β-estradiol - 17β استرادیولSplenocytes - اسپلنوسیت هاimmunoglobulin M - ایمونوگلوبولین MEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاCell–cell interaction - تعامل سلول-سلولfetal bovine serum - سرم جنین گاوPhosphate buffered saline - فسفات بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتlipopolysaccharide - لیپوپلی ساکاریدEstrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Estrogens have diverse effects on cell growth, differentiation and homeostatic functions, and have been shown to play an important role in regulating immune system. In this study, we examined the effect of 17β-estradiol (E2) on antibody production by splenocytes isolated from C57BL/6N mice. Our results suggest that the activation of immunoglobulin (Ig) M production by E2 requires direct cell-cell interaction between adherent and non-adherent cells in mouse splenocyte population, and the primary target of E2 is adherent cell population. In addition, we indicated that ER antagonist ICI 182780 suppressed this enhancing effect of E2. Both ERα agonist and ERα agonist enhanced IgM production of mouse splenocytes. ERs are expressed on plasma membrane as well as in nucleus. However, a plasma membrane-associated ER specific ligand has no stimulation effect on IgM production. In conclusion, our results indicate that adherent cells stimulated by E2 up-regulate IgM production of lymphocytes through the direct cell-cell interactions, and the enhancing effect of E2 is arouse through ERα and ERβ on these cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 98, Issue 2, 15 May 2005, Pages 225-231
Journal: Immunology Letters - Volume 98, Issue 2, 15 May 2005, Pages 225-231
نویسندگان
Mako Nakaya, Masao Yamasaki, Hirofumi Tachibana, Koji Yamada,