کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9266419 1217285 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-invasive live-cell measurement of changes in macrophage NAD(P)H by two-photon microscopy
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Non-invasive live-cell measurement of changes in macrophage NAD(P)H by two-photon microscopy
چکیده انگلیسی
Two-photon microscopy allows determination of UV-excitable fluorophores using long-wavelength light. We aimed to determine NAD(P)H autofluorescence as a measure for macrophage NADPH-oxidase activation. RAW264.7 macrophages were grown on glass coverslips and kept in HBSS for microscopic investigation. Cells were excited with 710 nm light and NAD(P)H autofluorescence was detected. Glucose as well as NaCN evoked an increase of NAD(P)H autofluorescence. Activators of NADPH oxidase lead to significantly decreased NAD(P)H autofluorescence. Therefore, this work shows the suitability of two-photon microscopy as a non-invasive method determining changes in phagocyte NAD(P)H upon activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 96, Issue 1, 15 January 2005, Pages 33-38
نویسندگان
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