کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9289334 | 1594411 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adenoviral expression of a truncated S1 subunit of SARS-CoV spike protein results in specific humoral immune responses against SARS-CoV in rats
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کلمات کلیدی
SARS-associated coronavirusPBSSPFAd-LacZPBSTSARS-CoVMOI - MEPBS containing 0.05% Tween 20 - PBS حاوی 0.05٪ Tween 20Enzyme-Linked Immuno-Sorbent Assay - آنزیم مرتبط با ایمونوسیبنیتهSpike - اسپایکHumoral immunity - ایمنی هومورالAdenoviral vector - بردار adenoviralELISA - تست الیزاspecific pathogen-free - خاص بدون پاتوژنSARS - سارسsevere acute respiratory syndrome - سندرم شدید حاد تنفسیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریplaque-forming unit - واحد پلاک سازیVaccine - واکسنpfu - پفوmultiplicity of infection - چندین عفونتSpike gene - ژن اسپایک
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The causative agent of severe acute respiratory syndrome (SARS) has been identified as SARS-associated coronavirus (SARS-CoV), but the prophylactic treatment of SARS-CoV is still under investigation. We constructed a recombinant adenovirus containing a truncated N-terminal fragment of the SARS-CoV Spike (S) gene (from â45 to 1469, designated Ad-SN), which encoded a truncated S protein (490 amino-acid residues, a part of 672 amino-acid S1 subunit), and investigated whether this construct could induce effective immunity against SARS-CoV in Wistar rats. Rats were immunized either subcutaneously or intranasally with Ad-SN once a week for three consecutive weeks. Our results showed that all of the immunized animals generated humoral immunity against the SARS-CoV spike protein, and the sera of immunized rats showed strong capable of protecting from SARS-CoV infection in vitro. Histopathological examination did not find evident side effects in the immunized animals. These results indicate that an adenoviral-based vaccine carrying an N-terminal fragment of the Spike gene is able to elicit strong SARS-CoV-specific humoral immune responses in rats, and may be useful for the development of a protective vaccine against SARS-CoV infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 112, Issues 1â2, September 2005, Pages 24-31
Journal: Virus Research - Volume 112, Issues 1â2, September 2005, Pages 24-31
نویسندگان
Ran-Yi Liu, Li-Zhi Wu, Bi-Jun Huang, Jia-Ling Huang, Yan-Ling Zhang, Miao-La Ke, Jun-Mei Wang, Wei-Ping Tan, Ru-Hua Zhang, Han-Kui Chen, Yi-Xin Zeng, Wenlin Huang,