Symbiotic effectiveness and bacteriocin production by Rhizobium leguminosarum bv. viciae isolated from agriculture soils in Faisalabad

It was isolated and partially purified to homogeneity by chloroform extraction followed by ammonium sulfate fractionation. The bacteriocin fraction consistently migrated as a 50 kDa polypeptide on SDS-PAGE. Ex vivo assays were carried out using the partially purified bacteriocin protein fraction of R. leguminosarum bv. viciae strain LC-31 against related strains of the same species. It showed an activity pattern similar to that exhibited by strain LC-31.