کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604243 | 43605 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of syntenin binding protein in the black tiger shrimp Penaeusmonodon
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کلمات کلیدی
SOX45-bromo-4-chloro-3-indolyl-α-d-galactopyranosideX-α-GalX-GalORFPDZPMSFWSSVα2M - a2malpha-2-macroglobulin - آلفا 2-ماگروگلوبولینImmune - ایمنیsynthetic dropout - ترک خوردگی مصنوعیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدPhosphotyrosine phosphatase - فسفاتیروزین فسفاتازopen reading frame - قاب خواندن بازyeast two-hybrid - مخمر دو رقمیRace - مسابقهPenaeus monodon - میگوی ببری بزرگsyntenin - همنشینWhite spot syndrome virus - ویروس سندرم سفید سفیدpostsynaptic density protein - پروتئین چگالی پست سیناپسی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and characterization of syntenin binding protein in the black tiger shrimp Penaeusmonodon Identification and characterization of syntenin binding protein in the black tiger shrimp Penaeusmonodon](/preview/png/9604243.png)
چکیده انگلیسی
Shrimp exhibit a diverse response to viral infection that is manifested in drastic up- and down-regulations of a variety of genes. In our previous work, we identified syntenin of the shrimp Penaeus monodon (Pm) as a dynamic responder to white spot syndrome virus (WSSV) infection, its message being greatly upregulated in the acute phase of the infection. In order to further explore the link between Pm-syntenin and viral infection, we performed a yeast two-hybrid screening of a P. monodon cDNA library, using Pm-syntenin as bait. One of the molecules that specifically interacted with Pm-syntenin was the receptor-binding domain of alpha-2-macroglobulin (α2M). A GST pull-down assay showed that GST-α2M, but not GST alone, was capable of co-precipitating syntenin. Another GST pull-down assay showed that GST-syntenin, but not GST alone, was capable of co-precipitating α2M. In addition, mutant analyses showed that the N-terminal 131 amino acids of syntenin were both necessary and sufficient to bind the C-terminus receptor-binding domain of α2M. Furthermore, WSSV-infected Pm showed a significant upregulation of the α2M message, suggesting that both syntenin and its protein partner α2M are upregulated in the acute phase of a WSSV infection. Taken together with a previous report showing the co-localization of α2M and syntenin in the exosome of a dendritic cell line, it is likely that syntenin, through its interaction with α2M, plays an important role in the immune defense mechanisms of viral infections of shrimps.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 120, Issue 2, 4 November 2005, Pages 135-145
Journal: Journal of Biotechnology - Volume 120, Issue 2, 4 November 2005, Pages 135-145
نویسندگان
Moltira Tonganunt, Amornrat Phongdara, Wilaiwan Chotigeat, Kenichi Fujise,