کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9720946 1473267 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Signal transduction pathways of group I metabotropic glutamate receptor-induced long-term depression at sensory spinal synapses
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Signal transduction pathways of group I metabotropic glutamate receptor-induced long-term depression at sensory spinal synapses
چکیده انگلیسی
Activation of spinal group I metabotropic glutamate receptors (mGluRs) may have antinociceptive or pro-nociceptive effects in different pain models. Pharmacological activation of group I mGluRs leads to long-term depression (LTD) of synaptic strength between Aδ-fibers and neurons in lamina II of spinal dorsal horn of the rat. Here, we studied the signal transduction pathways involved. Synaptic strength between Aδ-fibers and lamina II neurons was assessed by perforated whole-cell patch-clamp recordings in a spinal cord-dorsal root slice preparation of young rats. Bath application of the specific group I mGluR agonist (S)-3,5-dihydroxyphenylglycine [(S)-3,5-DHPG] produced an LTD of Aδ-fiber-evoked responses. LTD induction by (S)-3,5-DHPG was prevented, when intracellular Ca2+ stores were depleted by thapsigargin or cyclopiazonic acid (CPA). Preincubation with ryanodine to inhibit Ca2+-induced Ca2+ release had no effect on LTD-induction by (S)-3,5-DHPG. In contrast, pretreatment with 2-aminoethoxydiphenyl borate (2-APB), an inhibitor of inositol-1,4,5-trisphosphate (IP3)-sensitive Ca2+ stores prevented LTD induction. Preincubation with the specific protein kinase C (PKC) inhibitors bisindolylmaleimide I (BIM) or chelerythrine, respectively, had no effect. Inhibition of L-type VDCCs by verapamil or nifedipine prevented LTD-induction by (S)-3,5-DHPG. The presently identified signal transduction cascade may be relevant to the long-term depression of sensory information in the spinal cord, including nociception.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pain - Volume 118, Issues 1–2, November 2005, Pages 145-154
نویسندگان
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