Classification and replacement test of HPLC systems using principal component analysis

Replacement of chromatographic systems is possible on the basis of measured chromatographic parameters using principal component analysis. The monofunctional test compounds provide only approximate information on the usefulness, classification and replacement of HPLC systems, if the aim is the separation of multifunctional solutes. Although the theoretical plate number (column efficiency) is negatively correlated with the symmetry factor, both are necessary for proper classification and characterization of stationary phases.