کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9879410 | 1534757 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of Li+ binding sites and the effect of Li+ treatment on phospholipid composition in human neuroblastoma cells: a 7Li and 31P NMR study
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کلمات کلیدی
τcPEPRBCLithium - لیتیمsphingomyelin - اسفنگومیلینNMR - تشدید مغناطیسی هستهای Spin–lattice relaxation time - زمان آرام شدن اسپین-شبکهspin–spin relaxation time - زمان آرامش چرخش اسپینrotational correlation time - زمان همبستگی چرخشیCytosol - سیتوزولCell membrane - غشای سلولیphosphatidylinositol - فسفاتیدیل اینوزیتولphosphatidylcholine - فسفاتیدیل کولینphosphatidylethanolamine - فسفاتیدیلتانولامینPhosphatidylserine - فسفاتیدیلسرینPhospholipid - فسفولیپیدMitochondria - میتوکندریاred blood cell - گلبول قرمز، اریتروسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Li+ binding in subcellular fractions of human neuroblastoma SH-SY5Y cells was investigated using 7Li NMR spin-lattice (T1) and spin-spin (T2) relaxation measurements, as the T1/T2 ratio is a sensitive parameter of Li+ binding. The majority of Li+ binding occurred in the plasma membrane, microsomes, and nuclear membrane fractions as demonstrated by the Li+ binding constants and the values of the T1/T2 ratios, which were drastically larger than those observed in the cytosol, nuclei, and mitochondria. We also investigated by 31P NMR spectroscopy the effects of chronic Li+ treatment for 4-6 weeks on the phospholipid composition of the plasma membrane and the cell homogenate and found that the levels of phosphatidylinositol and phosphatidylserine were significantly increased and decreased, respectively, in both fractions. From these observations, we propose that Li+ binding occurs predominantly to membrane domains, and that chronic Li+ treatment alters the phospholipid composition at these membrane sites. These findings support those from clinical studies that have indicated that Li+ treatment of bipolar patients results in irregularities in Li+ binding and phospholipid metabolism. Implications of our observations on putative mechanisms of Li+ action, including the cell membrane abnormality, the inositol depletion and the G-protein hypotheses, are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1741, Issue 3, 25 September 2005, Pages 339-349
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1741, Issue 3, 25 September 2005, Pages 339-349
نویسندگان
Brian T. Layden, Abde M. Abukhdeir, Christopher Malarkey, Lisa A. Oriti, Wajeeh Salah, Claire Stigler, Carlos F.G.C. Geraldes, Duarte Mota de Freitas,